PXD061878-1

PXD061878 is an original dataset announced via ProteomeXchange.

Title	The MicroRNA pathway regulates obligatory aestivation in a flea beetle
Description	Aestivation is a dormant state that allows animals to withstand hot and dry summer conditions and requires complex gene regulation. Nevertheless, the mechanisms involved in the regulation of genes necessary for aestivation remain unclear. MicroRNA (miRNA) are known to fine-tune gene expression at the post-transcriptional level and are important for various biological processes. In this study, we investigated the role of the miRNA pathway in the regulation of the obligatory aestivation stage in the cabbage stem flea beetle, a major pest of oilseed rape. Small RNA sequencing showed that ∼25% of miRNAs were differentially abundant during aestivation. The inhibition of the miRNA pathway deregulated 116 proteins in aestivation, which were mainly associated with metabolism and catabolism, including peroxisome activity. Most proteins regulated by miRNA exhibited lower transcript levels during aestivation. RNA degradome sequencing confirmed the miRNA-mediated exonucleolytic decay of several transcripts. Furthermore, inhibiting the miRNA pathway resulted in altered body composition, compromised metabolic suppression, and lower resilience to high temperature during aestivation. Also, beetles could not suppress their feeding activity during the transition into aestivation. Our findings highlight the critical role of miRNA in regulating aestivation in the cabbage stem flea beetle, with important implications for climate change.
HostingRepository	PRIDE
AnnounceDate	2025-07-26
AnnouncementXML	Submission_2025-07-26_13:53:15.167.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Doga Cedden
SpeciesList	scientific name: Psylliodes chrysocephala; NCBI TaxID: 2598218;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-15 09:06:27	ID requested
⏵ 1	2025-07-26 13:53:15	announced

10.1101/2025.02.05.631883;

submitter keyword: LC-MSMS

Doga Cedden
contact affiliation	University of Göttingen, Dept. of Evolutionary Developmental Genetics, Germany
contact email	doga.cedden@biologie.uni-goettingen.de
lab head
Doga Cedden
contact affiliation	University of Göttingen
contact email	doga.cedden@biologie.uni-goettingen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061878
     1. Label: PRIDE project
     2. Name: The MicroRNA pathway regulates obligatory aestivation in a flea beetle