PXD061871-1

PXD061871 is an original dataset announced via ProteomeXchange.

Title	Chemoselective Characterization of New Extracellular Matrix Deposition in Bioengineered Tumor Tissues
Description	Investigating ECM-regulated new ECM production has been challenging due to the proteomic background from the pre-existing biomaterial ECM. To address this issue the newly synthesized ECM proteins (newsECM) of two different bioengineered tumor models were analyzed: tumor tissues, built upon decellularized ECM (dECM-tumors) or as ECM-free tumor spheroids (tumoroids). newsECM proteins were labeled and enriched using a chemoselective strategy, employing a click-chemistry compatible, azido tag bearing monosaccharide probe, azido-galactosamine tetra-acetylated (Ac4GalNAz). The newsECM profiles within the two tumor models were characterized and compared by means of LC-MS/MS and subsequent follow up data analysis.
HostingRepository	PRIDE
AnnounceDate	2025-08-30
AnnouncementXML	Submission_2025-08-30_13:41:20.453.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD061871
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Burke Niego
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-14 19:31:26	ID requested
⏵ 1	2025-08-30 13:41:20	announced

10.1002/ADMA.202505445;

10.6019/PXD061871;

submitter keyword: Chemoselective chemistry,Newly-synthesized extracellular matrix, Biomaterial, Proteomics

Lloyd M.
contact affiliation	Department of Chemistry, University of Wisconsin-Madison, United States
contact email	smith@chem.wisc.edu
lab head
Burke Niego
contact affiliation	University of Wisconsin-Madison
contact email	bniego@wisc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061871
     1. Label: PRIDE project
     2. Name: Chemoselective Characterization of New Extracellular Matrix Deposition in Bioengineered Tumor Tissues