PXD061861-1

PXD061861 is an original dataset announced via ProteomeXchange.

Title	Pleiotropic effects of Metformin on the chemotherapy response of HPV-positive cancer cells
Description	Metformin has been shown to downregulate viral E6/E7 expression in HPV-positive cancer cells. However, Metformin does not induce cellular senescence, which is observed upon E6/E7 repression by RNA interference. Here we compare mass spectrometry-based proteome analyses of Metformin-treated SiHa cells (previously deposited to the ProteomeXchange Consortium with data set identifier PXC011095) with proteome analyses of SiHa cells transfected with si16E6/E7 or control siRNA (siCtrl-1) and grown for 72 hours to evaluate differences in the cell cycle regulation of HPV-positive cancer cells upon E6/E7 repression by Metformin or siE6/E7.
HostingRepository	PRIDE
AnnounceDate	2025-06-17
AnnouncementXML	Submission_2025-06-17_13:55:23.272.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bianca Kuhn
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-14 13:11:06	ID requested
⏵ 1	2025-06-17 13:55:23	announced

10.1002/JMV.70434;

submitter keyword: human papillomavirus (HPV), cervical cancer, oncogenesis, Metformin, chemotherapy, oncoproteins

Felix Hoppe-Seyler
contact affiliation	Molecular Therapy of Virus-Associated Cancers, German Cancer Research Center (DKFZ), D-69120 Heidelberg, Germany
contact email	hoppe-seyler@dkfz-heidelberg.de
lab head
Bianca Kuhn
contact affiliation	Broad Institute of MIT and Harvard
contact email	bkuhn@broadinstitute.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD061861

PRIDE project URI

• PRIDE
  1. PXD061861
     1. Label: PRIDE project
     2. Name: Pleiotropic effects of Metformin on the chemotherapy response of HPV-positive cancer cells