PXD061853-1
PXD061853 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Integrated genomics and proteomics analysis uncovers the oxidative stress role of miR-10b targets in healthy and diabetic human limbal epithelial cells |
| Description | The corneal epithelium is maintained by limbal epithelial stem cells (LESCs) and is largely responsible for corneal optical transparency and protection by continuously renewing population of corneal epithelial cells. Diabetes mellitus (DM) affects all structures of the eye including the cornea, which can result in delayed wound healing and potential vision loss. MicroRNAs (miRNAs) are short non-coding oligonucleotides that regulate various cellular functions, including oxidative stress response, by repressing protein translation. MiR-10b-5p was previously identified to be upregulated in diabetic vs. non-diabetic limbal cells, and our purpose was to understand the role of miR-10b-5p in human limbal epithelial cells in healthy and diabetic conditions. Through integrated transcriptomic and proteomic analyses, we identified GCLM and LANCL1 as key miR-10b-5p targets, revealing its profound impact on glutathione metabolism, sulfur compound biosynthesis processes, and antioxidant defenses. Our findings suggest that overexpression of miR-10b disrupts redox balance, which potentially leads to heightened oxidative stress and increased cellular vulnerability in diabetic corneas. Understanding miR-10b function in corneal epithelial cells may pave the way for novel therapeutic strategies to mitigate oxidative stress and normalize corneal health in diabetic patients. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-07-10 |
| AnnouncementXML | Submission_2025-07-10_14:08:12.351.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Niveda Sundararaman |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-03-14 10:58:43 | ID requested | |
| ⏵ 1 | 2025-07-10 14:08:12 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: diabetic cornea, limbal stem cells, miR-10b, oxidative stress, DatasetType:Proteomics |
Contact List
| Mehrnoosh Saghizadeh Ghiam | |
|---|---|
| contact affiliation | Cedars-Sinai Medical Center |
| contact email | Mehrnoosh.ghiam@cshs.org |
| lab head | |
| Niveda Sundararaman | |
| contact affiliation | Cedars SInai Medical Center |
| contact email | Niveda.Sundararaman@cshs.org |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097328/ |
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