PXD061804 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | GlycoFASP: A Universal Method to Prepare Complex Mixtures for O-Glycoproteomic Analysis |
| Description | Here we describe a method titled filter-aided preparation of O-glycoproteomic samples (GlycoFASP) which takes advantage of molecular-weight cut-off filters and O-glycoprotease digestion specificity. Using GlycoFASP, preparation for MS analysis of biological samples is completed within one day and only requires one milligram of sample. It is both low-cost and amenable to non-specialists. GlycoFASP regularly achieves depletion of nearly all non-O-glycosylated proteins, with ≥70% of the protein signal originating from O-glycoproteins. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-07-08 |
| AnnouncementXML | Submission_2026-07-08_06:13:49.757.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Shane Finn |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | L-homoarginine; complex glycosylation; iodoacetamide derivatized residue |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-03-13 11:29:04 | ID requested | |
| ⏵ 1 | 2026-07-08 06:13:50 | announced | |
Publication List
| Finn SM, Mahoney KE, Lucas TM, Rangel-Angarita V, Chen RJ, Malaker SA, GlycoFASP: A Universal Method to Prepare Complex Mixtures for O-Glycoproteomic Analysis. Anal Chem, 97(43):23751-23756(2025) [pubmed] |
| 10.1021/acs.analchem.5c02666; |
Keyword List
| submitter keyword: Human, Method, Serum, LC-MS, Glycoproteomics, O-glycoproteomics, Seminal Fluid |
Contact List
| Stacy Malaker |
|---|
| contact affiliation | Yale University |
| contact email | Stacymalaker@gmail.com |
| lab head | |
| Shane Finn |
|---|
| contact affiliation | Yale University |
| contact email | shane.finn@yale.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD061804 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD061804
- Label: PRIDE project
- Name: GlycoFASP: A Universal Method to Prepare Complex Mixtures for O-Glycoproteomic Analysis
