PXD061800-1

PXD061800 is an original dataset announced via ProteomeXchange.

Title	Cellular assembly, functional hierarchy, and proteostatic control of the RNA exosome in mouse embryonic stem cells
Description	Most eukaryotic proteins are constituents of multiprotein complexes, yet our understanding of how individual subunits contribute to complex function, assembly and stability remains limited. Here, we utilize a doxycycline-inducible dual guide CRISPR/Cas9 system (iCas9) in mESCs to systematically investigate each component of the RNA exosome, an essential exoribonuclease complex with a central role in RNA processing and degradation. We report that depletion of individual RNA exosome components results in reciprocal proteolytic degradation of select complex subunits, revealing substantial proteostatic control mechanisms that oversees an ordered cellular assembly of the mouse RNA exosome. Phenotypic and functional analyses establish Exosc1 as a dispensable and peripheral component of the otherwise essential RNA exosome core. Our findings provide detailed insights into the cellular assembly, functions and proteostatic control of the mouse RNA exosome and offer an experimental framework to dissect other multisubunit protein complexes.
HostingRepository	PRIDE
AnnounceDate	2026-03-28
AnnouncementXML	Submission_2026-03-27_23:22:05.059.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Imre
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	phosphorylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse; Q Exactive HF; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-13 09:26:20	ID requested
⏵ 1	2026-03-27 23:22:05	announced

Dataset with its publication pending

submitter keyword: mESCs,RNA exosome, Exosome inducible knockouts, iCas9

Stefan Ludwig Ameres
contact affiliation	Univ.-Prof. Dr., University of Vienna, Max Perutz Labs, Vienna Biocenter Campus (VBC)
contact email	stefan.ameres@univie.ac.at
lab head
Richard Imre
contact affiliation	IMBA Vienna
contact email	richard.imre@imba.oeaw.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD061800

PRIDE project URI

• PRIDE
  1. PXD061800
     1. Label: PRIDE project
     2. Name: Cellular assembly, functional hierarchy, and proteostatic control of the RNA exosome in mouse embryonic stem cells