PXD061794-1

PXD061794 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Identification of p62-interacting proteins by mass spectrometry analysis in HEK293T cells
Description	HEK293T cells were ectopically expressing Flag tagged p62 (p62 group) or empty vectors (Control group) for 48h, cell lysates were incubated with anti-Flag affinity gels, and co-immunoprecipitates were subjected to trypsin digestion followed by mass spectrometry analysis. Peptides were separated by the EASY-nLC system (Thermo Fisher) and analyzed by the Q Exactive mass spectrometer (Thermo Fisher). Protein analysis were performed with Thermo Proteome Discoverer 2.1 (Thermo Fisher) and searched against Uniprot Human database. Three samples per group.
HostingRepository	PRIDE
AnnounceDate	2025-07-29
AnnouncementXML	Submission_2025-07-29_08:04:22.528.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	yun yang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-13 07:49:37	ID requested
⏵ 1	2025-07-29 08:04:23	announced

Publication List

Qiu X, Ren J, Yang Y, Hu W, Wang C, Hu R, Li C, TRIM25 ubiquitinates and degrades p62/SQSTM1 to suppress autophagy. Acta Biochim Biophys Sin (Shanghai), ():(2025) [pubmed]
10.3724/abbs.2025062;

Qiu X, Ren J, Yang Y, Hu W, Wang C, Hu R, Li C, TRIM25 ubiquitinates and degrades p62/SQSTM1 to suppress autophagy. Acta Biochim Biophys Sin (Shanghai), ():(2025) [pubmed]

10.3724/abbs.2025062;

Keyword List

submitter keyword: HEK293T cells,p62, mass spectrometry analysis

submitter keyword: HEK293T cells,p62, mass spectrometry analysis

Contact List

yang yun
contact affiliation	The Second Affiliated Hospital Zhejiang University School of Medicine
contact email	yyang@ucas.ac.cn
lab head
yun yang
contact affiliation	Hangzhou Institute for Advanced Study
contact email	yyang@ucas.ac.cn
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD061794
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD061794

PRIDE project URI

Repository Record List

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