PXD061791-1

PXD061791 is an original dataset announced via ProteomeXchange.

Title	DMXL1 interaction proteomics from U2OS cells infected with SidK
Description	To assess the effect of SidK expression on DMXL1 interaction with the V1 subunits of the V-ATPase, U2OS cells stably expressing DMXL1 tagged with mNeonGreen (mNG) were infected with lentivirus to express either mCherry or mCherry-SidK. Post-infection, cells were harvested to perform immunoprecipitation (IP) of DMXL1-mNG using magnetic beads coupled with a nanobody against mNG. IP samples were subsequently processed for TMT-MS analysis. As negative control, immunoprecipitation was also performed from parental U2OS cells expressing mCherry. Each condition was performed in triplicate.
HostingRepository	PRIDE
AnnounceDate	2025-04-22
AnnouncementXML	Submission_2025-04-22_10:25:10.740.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD061791
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Chan Lee
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-13 06:18:02	ID requested
⏵ 1	2025-04-22 10:25:11	announced

10.6019/PXD061791;

submitter keyword: Lysosome,DMXL1, V-ATPase, SidK, U2OS

J. Wade Harper
contact affiliation	Department of Cell Biology, Harvard Medical School, Boston, MA
contact email	wade_harper@hms.harvard.edu
lab head
Chan Lee
contact affiliation	Harvard Medical School
contact email	chan_lee@hms.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061791
     1. Label: PRIDE project
     2. Name: DMXL1 interaction proteomics from U2OS cells infected with SidK