PXD061784-1

PXD061784 is an original dataset announced via ProteomeXchange.

Title	The cardiac phospho-proteome during pressure overload in mice
Description	Trans-aortic constriction (TAC) is a widely used murine model to study pressure overload-induced cardiac hypertrophy and heart failure. Despite its high prevalence during aortic stenosis or chronic arterial hypertension, the global alterations in cardiac proteome and phospho-proteome dynamics following TAC remain incompletely characterised. Here, we present a comprehensive database of detailing the phospho-proteomic signature of the mouse heart one day and seven days after TAC. Utilising proteomic and phosphor-proteomic analyses, we identified and quantified thousands of proteins and phosphorylation sites, revealing hundreds of differential phosphorylation events that are significantly altered in the cardiac response to pressure overload. Our analysis highlights significant changes in pathways related to hypertrophic signalling, metabolic remodelling, contractile function, and the stress response. We also present proteomic data from the main cardiac cell types (endothelial cells, fibroblasts and cardiomyocytes) after TAC to reveal the cellular localization of the detected phosphoproteins. The dataset offers insights into temporal and site-specific phosphorylation events, facilitating the potential discovery of novel therapeutic targets and biomarkers. By making this resource publicly available, we aim to enable further exploration of the molecular basis of cardiac remodelling and advance translational research in heart failure.
HostingRepository	PRIDE
AnnounceDate	2025-05-26
AnnouncementXML	Submission_2025-05-26_03:54:09.051.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frank Stein
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-13 01:22:24	ID requested
⏵ 1	2025-05-26 03:54:09	announced

Dataset with its publication pending

submitter keyword: Heart failure, Cardiac remodelling, signalling, TAC, Phosphorylation

Joerg Heineke
contact affiliation	Department of Cardiovascular Physiology, European Center for Angioscience (ECAS), Medical Faculty Mannheim of Heidelberg University, Germany.German Center for Cardiovascular Research (DZHK), partner site Heidelberg/Mannheim.
contact email	joerg.heineke@medma.uni-heidelberg.de
lab head
Frank Stein
contact affiliation	EMBL
contact email	frank.stein@embl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD061784

PRIDE project URI

• PRIDE
  1. PXD061784
     1. Label: PRIDE project
     2. Name: The cardiac phospho-proteome during pressure overload in mice