⮝ Full datasets listing
PXD061772-1
PXD061772 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Cell-Type-Specific Surfaceome Profiling of 100-500 Isolated Cells using a Droplet-Based Magnetic Affinity Purification System |
| Description | Cell surface proteins (CSPs) represent an important source of biomarkers and therapeutic targets. However, due to the inherent sensitivity limitations of existing technologies, tissue and cell-type-specific surfaceomes remain poorly characterized, especially in the context of human diseases. Herein, we develop nanoMAPS (nanoscale Magnetic Affinity Purification System), a miniaturized proteomic sample preparation method for surfaceome profiling of as few as 100-500 cells (1000 to 100,000 times lower than existing technologies). We demonstrate that the miniaturization of magnetic beads-based affinity purification inside a single droplet can efficiently improve the recovery of surface proteins and reduce non-specific absorption of intracellular proteins. By applying nanoMAPS to human immune cells isolated from PBMCs, we demonstrate robust identification of both well-known cell-type-specific surface markers and candidate cell-type-specific proteins. We establish nanoMAPS as a promising platform to expand surface proteomics from cultured cells to primary cells isolated from patients or mouse models. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-08-17 |
| AnnouncementXML | Submission_2026-08-17_09:37:12.519.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Amanda Lorentzian |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Carbamidomethyl; Oxidation; Acetyl |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-03-12 15:22:04 | ID requested | |
| ⏵ 1 | 2026-08-17 09:37:13 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Surfaceomics, Cell Surface, PBMC, low-input, microPOTS, nanoMAPS, DatasetType:Proteomics |
Contact List
| Ying Zhu | |
|---|---|
| contact affiliation | Genentech Inc. |
| contact email | zhu.ying@gene.com |
| lab head | |
| Amanda Lorentzian | |
| contact affiliation | Genentech |
| contact email | lorenta1@gene.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097303/ |
