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PXD061762-1

PXD061762 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDynamic Organellar Mapping in unperturbed yeast
DescriptionSophisticated techniques are available for systematic studies of yeast cell biology. However, it remains challenging to investigate protein subcellular localization changes on a proteome-wide scale. Here, we apply Dynamic Organellar Maps (DOMs) by label-free mass spectrometry to detect localization changes of native, untagged proteins during endoplasmic reticulum (ER) stress. This dataset comprises 6 static maps generated using the newly established sample processing workflow for yeast cells. From this dataset we generated protein copy number estimates, cytosolic pool estimates and subcellular localization predictions for nearly 3000 proteins.
HostingRepositoryPRIDE
AnnounceDate2025-12-03
AnnouncementXMLSubmission_2025-12-03_09:54:44.728.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJulia Schessner
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932; scientific name: Saccharomyces cerevisiae; NCBI TaxID: NCBITaxon:4932;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-03-12 08:43:50ID requested
12025-12-03 09:54:45announced
Publication List
10.1038/S41467-025-66946-8;
Keyword List
submitter keyword: Dynamic Organellar Maps, Yeast, Subcellular localization
Contact List
Georg H H Borner
contact affiliationDepartment of Proteomics and Signal Transduction, Systems Biology of Membrane Trafficking Research Group, Max-Planck Institute of Biochemistry, Martinsried, Germany
contact emailborner@biochem.mpg.de
lab head
Julia Schessner
contact affiliationMax-Planck-Institute of Biochemistry
contact emailschessner@biochem.mpg.de
dataset submitter
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