PXD061756-1

PXD061756 is an original dataset announced via ProteomeXchange.

Title	A spatially resolved view on the aging substantia nigra: an exploratory proteomic study
Description	Physiological ageing is accompanied by numerous structural and molecular changes in the brain, with varying degrees in different brain areas, and is therefore considered one of the major risk factors for neurodegenerative diseases. As early pathomechanisms of neurodegeneration are often difficult to assess, analysing the ageing brain and its substructures may lead to insights into earliest alterations eventually leading to neurodegeneration. The present study focuses on elucidating age-related changes in the substantia nigra pars compacta, a brain region particularly vulnerable in Parkinson’s disease and its substructures. With a particular emphasis on neuromelanin granules we aimed to gain a spatially resolved view on aging-dependent alterations to draw conclusions on early processes potentially leading to neurodegeneration. Our findings, which included the identification of reduced levels of proteins involved in dopaminergic neurotransmission, suggest either a specific loss of dopaminergic neurons or a reduction in metabolic activity. Furthermore, we observed alterations in the abundances of mitochondrial proteins that are potentially indicative of an age-related shift in their localization within aged tissue donors. Consequently, the present exploratory study suggests that proteomic changes, frequently referred to as potential pathomechanisms of PD, namely dopaminergic dysfunction and aberrations in the mitochondrial population, are already occurring in the physiological aging process of the human SN
HostingRepository	PRIDE
AnnounceDate	2025-09-23
AnnouncementXML	Submission_2025-09-23_07:56:16.632.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Britta Eggers
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-12 05:15:18	ID requested
⏵ 1	2025-09-23 07:56:17	announced

10.1002/ADBI.202500358;

submitter keyword: neuromelanin granules, proteomics, parkinson’s disease,aging, dementia with Lewy bodies

Katrin Marcus
contact affiliation	Medizinisches Proteom-Center, Medical Faculty, Ruhr-University Bochum, Bochum, Germany
contact email	Katrin.marcus@rub.de
lab head
Britta Eggers
contact affiliation	Ruhr University Bochum
contact email	britta.eggers@rub.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD061756

PRIDE project URI

• PRIDE
  1. PXD061756
     1. Label: PRIDE project
     2. Name: A spatially resolved view on the aging substantia nigra: an exploratory proteomic study