PXD061737-1

PXD061737 is an original dataset announced via ProteomeXchange.

Title	Premature mesocarp vitality loss in Niagara Rosada occurs independently of cell death
Description	The grape berry mesocarp undergoes physiological and biochemical changes during ripening, including mesocarp cell death (MCD) and hydraulic isolation. We hypothesized that MCD does not occur in the Niagara Rosada (NR) variety but rather, a down-regulation of respiratory enzymes during the early loss of mesocarp cell viability while preserving membrane integrity. Hence, we analysed three developmental stages using label-free proteomics, enzymatic respiratory activity assays and outer mesocarp imaging. Cell wall-modifying proteins accumulated differentially throughout ripening, while cytoplasmic membranes remained intact. Additionally, the reduction in the mitochondria cristae density coincided with decreased malate dehydrogenase and succinate dehydrogenase activities. Proteomics analysis revealed 956 differentially accumulated proteins, with most down-regulated at ripening. However, the enzymes aldolase, glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase, enolase and malate dehydrogenase were among the most abundant at ripening or contributed significantly to data variance, suggesting the maintenance of respiratory activity despite mesocarp physiological changes. We also hypothesized that gluconeogenesis originated from malate occurs in mesocarp, and that sucrose futile cycles may play a crucial role in carbohydrate storing and unloading. Therefore, we suggest that the premature loss of berry mesocarp vitality in NR is not linked to cell death.
HostingRepository	PRIDE
AnnounceDate	2025-12-16
AnnouncementXML	Submission_2025-12-15_16:11:14.188.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ricardo Bressan-Smith
SpeciesList	scientific name: Vitis vinifera (Grape); NCBI TaxID: NEWT:29760;
ModificationList	No PTMs are included in the dataset
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-11 22:15:37	ID requested
⏵ 1	2025-12-15 16:11:14	announced

10.1021/ACSAGSCITECH.5C00280;

submitter keyword: mitochondrial dynamics, sucrose futile cycles, proteomics, carbohydrates metabolism, mesocarp cell viability,Grape berry ripening, respiratory enzymes activity

Ricardo Bressan-Smith
contact affiliation	Universidade Estadual do Norte Fluminense Darcy Ribeiro, Integrated Analysis of Plant Metabolism Laboratory, Campos dos Goytacazes, Brazil
contact email	bressan@uenf.br
lab head
Ricardo Bressan-Smith
contact affiliation	Universidade Estadual do Norte Fluminense
contact email	bressan@uenf.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD061737

PRIDE project URI

• PRIDE
  1. PXD061737
     1. Label: PRIDE project
     2. Name: Premature mesocarp vitality loss in Niagara Rosada occurs independently of cell death