PXD061714 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Mechanical cue regulates cargo sorting and export at the Golgi |
| Description | The secretory pathway is a sophisticated endomembrane machinery designed to transport and deliver proteins and lipids to intracellular organelles and the extracellular space. While the molecular components of the secretory pathway are well understood, less is known about their regulation, especially by mechanical cues. Here, we report that substrate stiffness stimulates conventional secretion. We have unravelled a molecular pathway that links a mechanical cue through Src and FAK kinases to promote the trafficking of secretory proteins out of the Golgi apparatus and prevent their post-Golgi lysosomal degradation. Phosphoproteomic analysis revealed the Golgi-specific Brefeldin A resistance factor 1 (GBF1) as a downstream key mechano-responsive regulator of post-Golgi sorting. Together, our data reveal a molecular regulatory loop in which the matrix stiffness positively regulates cellular secretion via the Src-FAK-GBF1 axis, which can have relevant medical implication in conditions like cancer and fibrosis and their treatment. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-01-12 |
| AnnouncementXML | Submission_2026-01-11_17:32:32.022.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Riccardo Rizzo |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | phosphorylated residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-03-11 11:34:09 | ID requested | |
| ⏵ 1 | 2026-01-11 17:32:33 | announced | |
Publication List
| 10.1002/advs.202506241; |
| Serafino G, Forciniti S, Scarpa E, Ranieri A, Santorelli L, De Blasi G, Costantini S, Lee EJ, Galeone A, Calcagn, ì A, Pirozzi M, Del Mercato LL, Venditti R, D'Angelo G, Parashuraman S, Verri T, Gigli G, Sztul ES, Grumati P, Rizzello L, Russo D, Rizzo R, Mechanical Cues Regulate Cargo Sorting and Export at the Golgi. Adv Sci (Weinh), 13(1):e06241(2026) [pubmed] |
Keyword List
| submitter keyword: GBF1, mechano'responsive,Golgi |
Contact List
| Riccardo Rizzo |
|---|
| contact affiliation | CNR |
| contact email | riccardo-rizzo@cnr.it |
| lab head | |
| Riccardo Rizzo |
|---|
| contact affiliation | Institute of Nanotechnology CNR-NANOTEC |
| contact email | riccardo-rizzo@cnr.it |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD061714
- Label: PRIDE project
- Name: Mechanical cue regulates cargo sorting and export at the Golgi
