PXD061666-1

PXD061666 is an original dataset announced via ProteomeXchange.

Title	Deep phenotyping of SH-Sy5y cells exposed to glucosylsphingosine by LC-MS
Description	SH-Sy5y cells were exposed to glucosylsphingosine (GlcSph) at two concentrations observed in moderate (20 ng/mL) and severe (200 ng/mL) Gaucher disease phenotypes and subjected to label free proteomics to identify cellular pathways and molecules affected by GlcSph. Lyso-Gb3, a lipid accumulating in Fabry disease, which exhibits no nerodegeneration, was used as a disease control. DMSO was used as a vehicle control.Proteomics analysis demonstrated a negative effect of GlcSph on cell metabolism, particularly affecting the TCA cycle, mitochondrial function, glycolysis and protein ubiquitination.
HostingRepository	PRIDE
AnnounceDate	2025-09-01
AnnouncementXML	Submission_2025-08-31_23:40:04.827.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD061666
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Valeria Nikolaenko
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-10 17:25:34	ID requested
⏵ 1	2025-08-31 23:40:05	announced

Nikolaenko V, Vootukuri R, Eaton S, H, ä, llqvist J, Baldwin T, Mills K, Heywood W, Glucosylsphingosine affects mitochondrial function in a neuronal cell model. Commun Biol, 8(1):1260(2025) [pubmed]

10.6019/PXD061666;

10.1038/s42003-025-08684-7;

submitter keyword: SH-Sy5y, LC-MS, glucosylsphingosine, Gaucher disease

Wendy Heywood
contact affiliation	Genetics and genomics medicine,Department of Population Health, UCL Institute of Child Health, London, UK
contact email	wendy.heywood@ucl.ac.uk
lab head
Valeria Nikolaenko
contact affiliation	UCL Institute of Child Health
contact email	sejjvni@ucl.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061666
     1. Label: PRIDE project
     2. Name: Deep phenotyping of SH-Sy5y cells exposed to glucosylsphingosine by LC-MS