PXD061658-1

PXD061658 is an original dataset announced via ProteomeXchange.

Title	Composition and RNA binding specificity of metazoan RNase MRP
Description	Mechanistic studies have revealed ribonuclease (RNase) MRP, a conserved RNA-based enzyme, is crucial for the maturation of ribosomal RNA in eukaryotes. However, the composition and RNA substrate specificity of this multisubunit ribonucleoprotein complex in higher eukaryotes remain unknown. Here, we identify NEPRO and C18ORF21 as constitutive subunits of metazoan RNase MRP. Both proteins are specific to RNase MRP and are the only ones distinguishing this enzyme from the closely related RNase P, which selectively cleaves transfer RNA-like substrates. We find that NEPRO and C18ORF21 each form a complex with all other subunits of RNase MRP, stabilize its catalytic RNA, and are required for rRNA maturation and cell proliferation. We harness our discovery to identify a full suite of in vivo RNA targets of each enzyme, including positions of potential cleavage sites at nucleotide resolution. In Summary, this project shows the general composition of metazoan RNase MRP, illuminate its RNA binding specificity, and provide valuable assets for functional exploration of this essential eukaryotic enzyme.
HostingRepository	PRIDE
AnnounceDate	2025-09-15
AnnouncementXML	Submission_2025-09-14_16:10:49.736.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	shanshan zheng
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-10 10:04:10	ID requested
⏵ 1	2025-09-14 16:10:50	announced

Liu Y, He S, Pyo K, Zheng S, Chen M, Braschi B, Cheloufi S, Slavov N, Marzluff WF, Murn J, Composition and RNA binding specificity of metazoan RNase MRP. Nucleic Acids Res, 53(16):(2025) [pubmed]

10.1093/nar/gkaf829;

submitter keyword: NEPRO,Ribonuclease (RNase) MRP, C18ORF21, IP-MS

Nikolai Slavov
contact affiliation	Departments of Bioengineering, Biology, Chemistry, and Chemical Biology, Single Cell Proteomics Center, and Barnett Institute, Northeastern University
contact email	n.slavov@northeastern.edu
lab head
shanshan zheng
contact affiliation	northeastern university
contact email	zheng.shan@northeastern.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061658
     1. Label: PRIDE project
     2. Name: Composition and RNA binding specificity of metazoan RNase MRP