PXD061524-1

PXD061524 is an original dataset announced via ProteomeXchange.

Title	The Plasmodium berghei merozoite protein PbMAP2 associates host erythrocyte binding and invasion
Description	The invasion of Plasmodium merozoites into host erythrocytes is initiated through specific ligand–receptor interactions. This interaction results in subsequent invasion events, facilitated by forming a moving junction through AMA–1 and associated molecular complexes mediating parasite entry into the erythrocytic membrane. Previous studies have implicated erythrocyte surface glycosaminoglycans, particularly heparan sulfate proteoglycans, as critical receptor components in this invasion process. In this study, we sought to elucidate the molecular function of PbMAP2 (encoded by PbANKA_1137800), a previously uncharacterized Plasmodium berghei ANKA protein, in mediating merozoite attachment and invasion via heparan sulfate–dependent pathways. The PbMAP2 protein, predominantly situated at the extreme apical region of the P. berghei merozoite, binds to heparin and the erythrocyte surface during merozoite invasion. Additionally, mice either immunized with the PbMAP2 protein or passively immunized with sera derived from vaccinated mice demonstrated enhanced immunity against lethal challenges. Global mass spectrometric analysis indicates that PbMAP2 engages with several trafficking and secretion proteins associated with parasite invasion. Our findings pinpointed PbMAP2 is predominantly expressed at the extreme apical region of the P. berghei merozoite and engages in binding to the heparin–like receptors on the erythrocyte surface during merozoite invasion.
HostingRepository	iProX
AnnounceDate	2025-03-06
AnnouncementXML	Submission_2025-03-05_22:47:07.222.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Junying Gao
SpeciesList	scientific name: Plasmodium sp. KYS10; NCBI TaxID: 1002265;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-05 22:46:56	ID requested
⏵ 1	2025-03-05 22:47:07	announced

Dataset with its publication pending

submitter keyword: Plasmodium, heparin–binding protein, invasion, dynamic, pathogenicity

Qijun Chen
contact affiliation	Shenyang Agricultural University
contact email	qijunchen759@syau.edu.cn
lab head
Junying Gao
contact affiliation	Shenyang Agricultural University
contact email	junyinggao@stu.syau.edu.cn
dataset submitter

iProX dataset URI