⮝ Full datasets listing

PXD061524-1

PXD061524 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe Plasmodium berghei merozoite protein PbMAP2 associates host erythrocyte binding and invasion
DescriptionThe invasion of Plasmodium merozoites into host erythrocytes is initiated through specific ligand–receptor interactions. This interaction results in subsequent invasion events, facilitated by forming a moving junction through AMA–1 and associated molecular complexes mediating parasite entry into the erythrocytic membrane. Previous studies have implicated erythrocyte surface glycosaminoglycans, particularly heparan sulfate proteoglycans, as critical receptor components in this invasion process. In this study, we sought to elucidate the molecular function of PbMAP2 (encoded by PbANKA_1137800), a previously uncharacterized Plasmodium berghei ANKA protein, in mediating merozoite attachment and invasion via heparan sulfate–dependent pathways. The PbMAP2 protein, predominantly situated at the extreme apical region of the P. berghei merozoite, binds to heparin and the erythrocyte surface during merozoite invasion. Additionally, mice either immunized with the PbMAP2 protein or passively immunized with sera derived from vaccinated mice demonstrated enhanced immunity against lethal challenges. Global mass spectrometric analysis indicates that PbMAP2 engages with several trafficking and secretion proteins associated with parasite invasion. Our findings pinpointed PbMAP2 is predominantly expressed at the extreme apical region of the P. berghei merozoite and engages in binding to the heparin–like receptors on the erythrocyte surface during merozoite invasion.
HostingRepositoryiProX
AnnounceDate2025-03-06
AnnouncementXMLSubmission_2025-03-05_22:47:07.222.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJunying Gao
SpeciesList scientific name: Plasmodium sp. KYS10; NCBI TaxID: 1002265;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-03-05 22:46:56ID requested
12025-03-05 22:47:07announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Plasmodium, heparin–binding protein, invasion, dynamic, pathogenicity
Contact List
Qijun Chen
contact affiliationShenyang Agricultural University
contact emailqijunchen759@syau.edu.cn
lab head
Junying Gao
contact affiliationShenyang Agricultural University
contact emailjunyinggao@stu.syau.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI