PXD061524-1
PXD061524 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | The Plasmodium berghei merozoite protein PbMAP2 associates host erythrocyte binding and invasion |
Description | The invasion of Plasmodium merozoites into host erythrocytes is initiated through specific ligand–receptor interactions. This interaction results in subsequent invasion events, facilitated by forming a moving junction through AMA–1 and associated molecular complexes mediating parasite entry into the erythrocytic membrane. Previous studies have implicated erythrocyte surface glycosaminoglycans, particularly heparan sulfate proteoglycans, as critical receptor components in this invasion process. In this study, we sought to elucidate the molecular function of PbMAP2 (encoded by PbANKA_1137800), a previously uncharacterized Plasmodium berghei ANKA protein, in mediating merozoite attachment and invasion via heparan sulfate–dependent pathways. The PbMAP2 protein, predominantly situated at the extreme apical region of the P. berghei merozoite, binds to heparin and the erythrocyte surface during merozoite invasion. Additionally, mice either immunized with the PbMAP2 protein or passively immunized with sera derived from vaccinated mice demonstrated enhanced immunity against lethal challenges. Global mass spectrometric analysis indicates that PbMAP2 engages with several trafficking and secretion proteins associated with parasite invasion. Our findings pinpointed PbMAP2 is predominantly expressed at the extreme apical region of the P. berghei merozoite and engages in binding to the heparin–like receptors on the erythrocyte surface during merozoite invasion. |
HostingRepository | iProX |
AnnounceDate | 2025-03-06 |
AnnouncementXML | Submission_2025-03-05_22:47:07.222.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Junying Gao |
SpeciesList | scientific name: Plasmodium sp. KYS10; NCBI TaxID: 1002265; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2025-03-05 22:46:56 | ID requested | |
⏵ 1 | 2025-03-05 22:47:07 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Plasmodium, heparin–binding protein, invasion, dynamic, pathogenicity |
Contact List
Qijun Chen | |
---|---|
contact affiliation | Shenyang Agricultural University |
contact email | qijunchen759@syau.edu.cn |
lab head | |
Junying Gao | |
contact affiliation | Shenyang Agricultural University |
contact email | junyinggao@stu.syau.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |