PXD061508-1

PXD061508 is an original dataset announced via ProteomeXchange.

Title	Pollen coat proteins involved in pathogen response show evidence of convergent evolution in independent transitions to selfing in Arabidopsis and Capsella species
Description	Convergent evolution, the independent evolution of similar traits in different species under similar environmental pressures, is widespread across many taxa. In plants, a key example is the repeated evolution of floral traits associated with the transition from outcrossing to self- fertilization, often resulting in the “selfing syndrome” (e.g., reduced flower size, loss of scent, and altered reproductive strategies). However, potentially overlooked changes concern the pollen coat, which plays a role in different aspects of outcrossing strategy. To investigate this, we compared pollen morphology and proteomes between selfing and outcrossing species in the 2 Arabidopsis and Capsella genera, representing three independent selfing transitions. Pollen coat area diminished with the age of the selfing transition, with significant reductions in A. thaliana and Capsella rubella, but not in A. lyrata (recent transition, no selfing syndrome). Moreover, differentially expressed pollen coat proteins, primarily related to pathogen response, were shared across selfer-outcrosser contrasts more than expected by chance. However, similar functions appeared in random outcrosser-outcrosser comparisons, suggesting additional drivers beyond mating system transitions. Overall, our findings reveal convergent evolution of pollen coat morphology and protein composition following selfing transitions, while highlighting pathogen response as a key factor driving the fast evolution of pollen coat protein independently of the mating system.
HostingRepository	PRIDE
AnnounceDate	2026-04-08
AnnouncementXML	Submission_2026-04-08_03:07:43.793.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pavel Talacko
SpeciesList	scientific name: Arabidopsis lyrata; NCBI TaxID: NEWT:59689; scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702; scientific name: Capsella rubella; NCBI TaxID: NEWT:81985; scientific name: Capsella grandiflora; NCBI TaxID: NEWT:264402; scientific name: Arabidopsis arenosa; NCBI TaxID: NEWT:38785;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-05 09:03:56	ID requested
⏵ 1	2026-04-08 03:07:44	announced

10.1111/NPH.71167;

submitter keyword: selfing transition, proteomics, pollen coat, pathogen response,convergence

Clément Lafon Placette
contact affiliation	Department of Botany, Faculty of Science, Charles University, CZ-128 01 Prague, Czech Republic
contact email	lafonplc@natur.cuni.cz
lab head
Pavel Talacko
contact affiliation	BIOCEV, Faculty of Science, Charles University in Prague
contact email	pavel.talacko@natur.cuni.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061508
     1. Label: PRIDE project
     2. Name: Pollen coat proteins involved in pathogen response show evidence of convergent evolution in independent transitions to selfing in Arabidopsis and Capsella species