PXD061491-1

PXD061491 is an original dataset announced via ProteomeXchange.

Title	Immunoprecipitation and mass spectrometry (IP-MS) on KAT6A chimeras
Description	Despite KAT6A chimeras potent oncogenic activity, the mechanisms by which KAT6A fusion proteins achieve genomic specificity and drive leukemogenesis remain poorly understood. Previous studies suggest that the leukemogenic potential of KAT6A-TIF2 depends on its chromatin-binding ability rather than its histone acetyltransferase (HAT) activity, with oncogenicity further amplified by CBP recruitment through its fusion partners. The winged helix domain (WH1) of KAT6A can bind unmethylated CpG islands and interact with P300/CBP via the TAZ2 domain. However, the widespread presence of unmethylated CpG islands across the genome does not fully explain the genomic specificity of KAT6A-CBP (K/C) and KAT6A-P300 (K/P) fusions, leaving key mechanistic gaps unexplored. To uncover protein interactions underlying these genomic associations, we performed immunoprecipitation and mass spectrometry (IP-MS) on KAT6A N-terminus and chimeras. Mass spectrometry analysis was performed to identify the core components of the KAT6A complex and the interacting proteins of KAT6A.
HostingRepository	PRIDE
AnnounceDate	2025-08-08
AnnouncementXML	Submission_2025-08-08_00:26:35.219.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Junhui Lv
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-04 23:40:12	ID requested
⏵ 1	2025-08-08 00:26:35	announced

Dataset with its publication pending

submitter keyword: Human, IP-MS., KAT6A fusion, Immunoprecipitation, KAT6A N-terminus

Kaiwei Liang
contact affiliation	Department of pathophysiology, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China
contact email	KWliang@whu.edu.cn
lab head
Junhui Lv
contact affiliation	Wuhan University
contact email	lvjunhui@whu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD061491

PRIDE project URI

• PRIDE
  1. PXD061491
     1. Label: PRIDE project
     2. Name: Immunoprecipitation and mass spectrometry (IP-MS) on KAT6A chimeras