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PXD061424-1

PXD061424 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Interactors and targeted substrates of UBL7 in mouse testis and sperm
Description	Spermiogenesis is a tightly regulated process to produce mature sperm cells. The ubiquitin-proteasome system (UPS) plays a crucial role in controlling protein half-life and is essential for spermiogenesis. In this study, we demonstrate that UBL7, a testis-enriched UBL-UBA protein, is indispensable for sperm formation. UBL7 interacts with the valosin-containing protein (VCP) complex and proteasomes, and shuttles substrates between them. Notably, UBL7 slows down the degradation rates of substrates involved in endoplasmic reticulum-associated degradation (ERAD) within cells. Through tandem mass tag (TMT)-labeled quantitative proteomics of sperm from Ubl7+/- and Ubl7-/- epididymis, we found lots of proteins were decreased in Ubl7-/- sperm, including critical factors involved in ATP metabolism and glycolytic process. Through a two-step immunoprecipitation method, we identify several essential factors in spermatids that are protected by UBL7, including factors involved in the development of manchette (such as IFT140), head-tail coupling apparatus (such as SPATA20) and cytoplasmic droplets (such as HK1 and SLC2a3). In summary, our findings highlight UBL7 as a guardian that protects crucial factors from excessive degradation and thereby ensures successful spermiogenesis.
HostingRepository	iProX
AnnounceDate	2025-03-04
AnnouncementXML	Submission_2025-03-31_20:08:54.172.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tianyi Yuan
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos; Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-03 19:32:28	ID requested
⏵ 1	2025-03-31 20:08:54	announced
2	2025-05-19 00:23:43	announced	2025-05-19: Add Publication.

Publication List

Dataset with its publication pending

Keyword List

submitter keyword: UBL7, sperm, UBL-UBA protein

Contact List

Fengchao Wang
contact affiliation	National Institute of Biological Sciences, Beijing
contact email	wangfengchao160415@163.com
lab head
Tianyi Yuan
contact affiliation	National Institute of Biological Sciences, Beijing
contact email	yuantianyi@nibs.ac.cn
dataset submitter

Full Dataset Link List

iProX dataset URI