PXD061409-1

PXD061409 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometric detection of keratins in tear fluid
Description	Keratin contamination is a common problem in mass spectrometry proteomic analyses, particularly in bottom-up mass spectrometry. The purpose of this study was to determine the protein contaminants introduced during the proteomic analysis of tear fluid.Human tear fluid samples were collected using Schirmer strips. Proteomic analyses were performed using liquid chromatography–tandem mass spectrometry (LC-MS/MS) on blank Schirmer strips and tear fluid samples, with empty vials serving as controls for assessing environmental contaminant proteins.This study revealed nine major contaminant keratins in the mass spectrometry analysis. Several other keratins were identified as constituents of tear fluid. Background subtraction is necessary for the accurate analysis of tear fluid using mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2025-03-03
AnnouncementXML	Submission_2025-03-03_10:31:19.007.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Saleh Ahmed
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-03 09:59:54	ID requested
⏵ 1	2025-03-03 10:31:19	announced

10.1016/j.exer.2025.110231;

Ahmed S, Altman J, Jones G, Lee TJ, Robertson DM, Zhi W, Sharma S, Sharma A, Mass spectrometric detection of keratins in tear fluid. Exp Eye Res, 251():110231(2025) [pubmed]

submitter keyword: Tear fluid, Keratins, Schirmer strip, Mass spectrometry, Proteomics, Biomarkers

Ashok Sharma
contact affiliation	Center for Biotechnology & Genomic Medicine, Department of Ophthalmology, Medical College of Georgia, Augusta University, USA
contact email	assharma@augusta.edu
lab head
Saleh Ahmed
contact affiliation	Medical College of Georgia, Augusta University
contact email	salahmed@augusta.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061409
     1. Label: PRIDE project
     2. Name: Mass spectrometric detection of keratins in tear fluid