PXD061406-1

PXD061406 is an original dataset announced via ProteomeXchange.

Title	A SMG7-eIF4A module constitutes a homeostatic system for P-bodies and Meiotic bodies
Description	The nucleation of RNP granules requires the participation of mRNAs, which simultaneously as becoming their target, dictate their properties. Here we report that in Arabidopsis the RNA helicase eIF4A limits the condensation of P-bodies acting as an RNA chaperone, disassembled from the eIF4F complex. eIF4A is recruited into P-bodies by SMG7, previously known to recruit UPF1 and TDM1. In response to heat shock (HS) eIF4A shares distribution within stress granules (SGs) and P-bodies, modulating the condensation of both RNP granules. Our study of meiotic RNP granules demonstrates the nucleation of the meiotic body (M-body), constituted by a P-body core and a SGs surrounding shell. SMG7 recruits eIF4A in the M-body core to modulate its condensation, which impacts the functionality of this body an influence plant reproduction. We conclude that SMG7 governs the functionality of P-bodies and M-bodies, and with eIF4A, constitutes a homeostatic system to control their level of condensation
HostingRepository	PRIDE
AnnounceDate	2026-03-25
AnnouncementXML	Submission_2026-03-25_10:08:08.986.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD061406
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Potesil
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-03 09:03:01	ID requested
⏵ 1	2026-03-25 10:08:09	announced

10.6019/PXD061406;

submitter keyword: eIF4A, stress granules, P-bodies, eIF4F, RNP granules, biomolecular condensates,SMG7, RNA condensation, meiosis, meiotic bodies

Zbynek Zdrahal
contact affiliation	Proteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic
contact email	zdrahal@sci.muni.cz
lab head
David Potesil
contact affiliation	Proteomics Core Facility and Research Group Proteomics CEITEC-MU Masaryk University Brno, Czech Republic
contact email	david.potesil@ceitec.muni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD061406

PRIDE project URI

• PRIDE
  1. PXD061406
     1. Label: PRIDE project
     2. Name: A SMG7-eIF4A module constitutes a homeostatic system for P-bodies and Meiotic bodies