PXD061314-1

PXD061314 is an original dataset announced via ProteomeXchange.

Title	Comparative shotgun proteomics analysis of wheat gluten proteins digested by various peptidases
Description	In this study, nine wheat cultivars were digested with five different peptidases or combinations. Large differences already occurred in the number of identified peptides. With 4115 peptides, trypsin resulted in by far the most peptide identifications, followed by thermolysin with 1421 peptides. However, this no longer applied at protein level. Although most metabolic protein groups (673) were identified with trypsin, this did not apply to gluten proteins of which around 130 gluten protein groups were identified with each peptidase. The ω-gliadins were detected with all peptidases except trypsin. A relative quantification of the gluten protein groups and comparison with quantitative RP-UHPLC-UV results showed that there was the greatest overlap after thermolysin digestion. Otherwise, there was not much overlap between the relatively quantified LC-MS/MS data of the different peptidases.
HostingRepository	PRIDE
AnnounceDate	2025-05-30
AnnouncementXML	Submission_2025-05-30_06:27:36.135.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christine Kaemper
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-28 09:27:08	ID requested
⏵ 1	2025-05-30 06:27:36	announced

10.1016/J.CRFS.2025.101095;

submitter keyword: Glutenin, Protease, Bottom-up proteomics, Gliadin,Triticum aestivum, Protein group

Katharina Anne
contact affiliation	Leibniz Institute for Food Systems Biology at the Technical University of Munich
contact email	k.scherf.leibniz-lsb@tum.de
lab head
Christine Kaemper
contact affiliation	Karlsruhe Institute of Technology
contact email	christine.kaemper@kit.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD061314

PRIDE project URI

• PRIDE
  1. PXD061314
     1. Label: PRIDE project
     2. Name: Comparative shotgun proteomics analysis of wheat gluten proteins digested by various peptidases