⮝ Full datasets listing
PXD061294-1
PXD061294 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Protein expression in pancreatic cancer cell lines and normal pancreatic ductal cell line. |
| Description | Pancreatic cancer (PC) is the sixth leading cause of cancer-associated death worldwide. Despite improvements in treatment methods, the 5-year survival rate of PC patients is still low. Although carbohydrate antigen (CA) 19-9 has been used as the most specific and sensitive biomarker for the diagnosis of PC, approximately 34% of PC patients may have a Lewis antigen-negative phenotype and secrete little, if any, CA19-9. Therefore, it is important to develop effective diagnostic methods for PC. In this study, we performed a global shotgun proteomics analysis using PC cell lines and a normal pancreatic ductal cell line. We identified 142 candidate proteins with differential expression in PC cells. A label-free semiquantitative method based on spectral counting and Gene Ontology analysis led to a total of 25 candidate proteins that could potentially be detected in blood. Validation studies revealed that the expression of fibulin-1 was lower in PC cells than in normal pancreatic ductal cells. Moreover, in vivo fibulin-1 expression was significantly lower in serum from PC patients than in healthy individuals. These findings suggest that lower blood levels of fibulin-1 may be a novel biomarker for the detection of PC. |
| HostingRepository | jPOST |
| AnnounceDate | 2025-12-03 |
| AnnouncementXML | Submission_2025-12-02_15:48:07.747.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | TETSUSHI YAMAMOTO |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide |
| Instrument | LTQ |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-02-27 19:10:52 | ID requested | |
| ⏵ 1 | 2025-12-02 15:48:08 | announced |
Publication List
| Yamamoto T, Boku S, Mitamura K, Taga A, Shotgun Label-Free Proteomic Analysis for Identification of a Potential Diagnostic Biomarker for Pancreatic Cancer. Biomedicines, 13(11):(2025) [pubmed] |
Keyword List
| submitter keyword: pancreatic cancer, shotgun proteomics, protein, diagnosis marker |
Contact List
| Tetsushi Yamamoto | |
|---|---|
| lab head | |
| TETSUSHI YAMAMOTO | |
| contact affiliation | Kindai University |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003631/ |
