PXD061284-1

PXD061284 is an original dataset announced via ProteomeXchange.

Title	Human cytomegalovirus induces neuronal gene expression through IE1 for viral maturation
Description	Viral invasion of the host cell causes some of the most dramatic changes in biology. Human cytomegalovirus (HCMV) extensively remodels host cells, altering nuclear shape and generating a cytoplasmic viral-induced assembly compartment (vIAC). How these striking morphology changes occur in the context of host gene regulation is still emerging. Here, we discovered that histone variant macroH2A1 is necessary for formation of infectious progeny and HCMV-induced reorganization of the host cell. Using RNA-seq we found that while all viral genes were highly expressed in the absence of macroH2A1, many HCMV-induced host genes were not. Remarkably, hundreds of these HCMV-induced macroH2A1-dependent host genes are associated with neuronal synapse formation and vesicle trafficking. Further, we found that HCMV immediate early protein, IE1, is sufficient to induce these neuronal genes, providing a mechanism of activation. Together, our findings demonstrate that HCMV hijacks a dormant neuronal secretory pathway through chromatin manipulation for efficient virion maturation.
HostingRepository	PRIDE
AnnounceDate	2026-02-09
AnnouncementXML	Submission_2026-02-08_16:09:09.912.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD061284
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alex Kentsis
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Cytomegalovirus; NCBI TaxID: NEWT:10358;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-27 14:18:21	ID requested
⏵ 1	2026-02-08 16:09:11	announced

10.1038/s41467-025-61915-7;

10.6019/PXD061284;

Kelnhofer-Millevolte LE, Smith JR, Nguyen DH, Wilson LS, Lewis HC, Arnold EA, Brinkley MR, Shin K, Ahn JH, Kim ET, Kulej K, Geballe AP, Ramachandran S, Avgousti DC, Human cytomegalovirus induces neuronal gene expression through IE1 for viral maturation. Nat Commun, 16(1):7316(2025) [pubmed]

submitter keyword: Human cytomegalovirus (HCMV), macroH2A1, histone PTMs, chromatin

Alex Kentsis, MD, PhD
contact affiliation	Tow Center for Developmental Oncology, Department of Pediatrics, Memorial Sloan Kettering Cancer Center, New York, NY, USA. Molecular Pharmacology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, USA. Departments of Pediatrics, Pharmacology, and Physiology & Biophysics, Weill Cornell Medical College, Cornell University, New York, NY, USA.
contact email	kentsisresearchgroup@gmail.com
lab head
Alex Kentsis
contact affiliation	Memorial Sloan Kettering Cancer Center
contact email	kentsisresearchgroup@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD061284

PRIDE project URI

• PRIDE
  1. PXD061284
     1. Label: PRIDE project
     2. Name: Human cytomegalovirus induces neuronal gene expression through IE1 for viral maturation