PXD061228-1

PXD061228 is an original dataset announced via ProteomeXchange.

Title	Intact MS of tubulin from murine embryonic stem cells
Description	Mitotic spindles assemble in a crowded cytoplasm whose physical properties can change during functional transitions between cell states. How cytoplasmic properties influence spindle architecture and scaling remains unknown. Spindle scaling relationships have been described in early animal development when cell volumes reduce dramatically. However, cells in these early developmental stages are transcriptionally inactive and rely on the cytoplasmic composition of the fertilised egg. By contrast, it is unknown whether and how differentiating cells in later stages of development adjust spindle architecture, specifically during brain development when spindle defects have severe pathological consequences. Using automated, non-invasive microscopy and three-dimensional analyses, we show concerted changes in cell and spindle morphology of murine embryonic stem cells undergoing neural differentiation. Remarkably, while tubulin biochemistry and microtubule dynamics remain unchanged, spindles in early-differentiated cells are smaller than spindles in equally-sized undifferentiated stem cells. Using quantitative theory and biophysical perturbations in combination with optical diffraction tomography, we find that differentiation-driven cytoplasmic dilution and a decrease in free tubulin concentration drive a CPAP-dependent increase in centrosomal nucleation capacity. As a consequence, in early-differentiated cells, microtubule mass is redistributed towards the spindle poles at the expense of the spindle bulk. For the first time, our study links cell-type specific cytoplasmiclle material properties to mitotic organelle morphology and scaling.
HostingRepository	PRIDE
AnnounceDate	2025-02-28
AnnouncementXML	Submission_2025-02-27_23:55:27.480.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Benno Kuropka
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-26 06:50:36	ID requested
⏵ 1	2025-02-27 23:55:27	announced

Dataset with its publication pending

submitter keyword: Mouse, Intact MS, Tubulin

Simone Reber
contact affiliation	Max Planck Institute for Infection Biology, Berlin, Germany
contact email	reber@mpiib-berlin.mpg.de
lab head
Benno Kuropka
contact affiliation	Freie Universität Berlin, Institute of Chemistry and Biochemistry, Laboratory of Protein Biochemistry, Thielallee 63, D-14195, Berlin, Germany
contact email	kuropka@zedat.fu-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD061228

PRIDE project URI

• PRIDE
  1. PXD061228
     1. Label: PRIDE project
     2. Name: Intact MS of tubulin from murine embryonic stem cells