PXD061227-1

PXD061227 is an original dataset announced via ProteomeXchange.

Title	A comparison of human skeletal muscle cell maturation in 2D versus 3D culture: A quantitative proteomic study C4PR_LIV
Description	Background Three-dimensional (3D) bioengineered models of human skeletal muscle are a promising approach for studying muscle development, function and disease in vitro. These models more closely resemble the complexity of native muscle tissue than two-dimensional (2D) monolayer culture and allow for functional measurements to be performed. However, a more complete understanding of how culture condition and duration impacts the myotube maturity and function is required to validate the transition from 2D to 3D culture of muscle cells. Methods Human skeletal muscle cells were cultured as either 2D monolayers or within 3D fibrin-based hydrogels as muscle constructs for up to 21 days. Quantitative proteomic analysis and functional assessments, including contractile force and cross-sectional area measurements, were conducted to evaluate the impact of culture conditions and duration on muscle cell differentiation. Results Proteomic analysis revealed myoblasts differentiated into myotubes by 8 days of differentiation in both 2D and 3D environments. However, the proteomic profiles of myotubes varied significantly between the two culture environments. At day 8 of differentiation, myosin heavy chain isotype abundance indicated a predominantly slow-twitch phenotype in 3D constructs, compared to a mixed fibre type phenotype in 2D monolayers. By day 21 of differentiation, 3D muscle constructs displayed improved mitochondrial maturity, extracellular matrix remodelling, and signs of transitioning towards a fast-twitch phenotype. This prolonged culture duration also resulted in increased passive tension but decreased peak contractile force in 3D muscle constructs. Conclusions This study demonstrates that 3D culture promotes maturity in human skeletal muscle cells, mimicking the biochemical cues and energy demands seen in native muscle tissue. The data highlights the importance of selecting appropriate culture conditions and durations when studying skeletal muscle cells in vitro and suggests 8 days of differentiation as optimal for achieving peak contractile force in 3D muscle constructs.
HostingRepository	PRIDE
AnnounceDate	2025-06-23
AnnouncementXML	Submission_2025-06-22_16:49:21.598.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Philip Brownridge
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-26 06:33:05	ID requested
⏵ 1	2025-06-22 16:49:22	announced

Tollitt BR, Jones SW, Ohana J, Henstock JR, Jackson MJ, McArdle A, A comparison of human skeletal muscle cell maturation in 2D versus 3D culture: A quantitative proteomic study. Physiol Rep, 13(12):e70420(2025) [pubmed]

10.14814/phy2.70420;

submitter keyword: Bioengineering, In vitro models, Skeletal muscle, Maturation, Proteomics, 3D culture, Hydrogel

Professor Anne McArdle
contact affiliation	Institute of Life Course & Medical Sciences Faculty of Health and Life Sciences University of Liverpool Liverpool UK
contact email	mdcr02@liverpool.ac.uk
lab head
Philip Brownridge
contact affiliation	University of Liverpool
contact email	philipjb@liv.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061227
     1. Label: PRIDE project
     2. Name: A comparison of human skeletal muscle cell maturation in 2D versus 3D culture: A quantitative proteomic study C4PR_LIV