PXD061221-1

PXD061221 is an original dataset announced via ProteomeXchange.

Title	NAD Depletion in Skeletal Muscle does not Compromise Muscle Function or Accelerate Aging
Description	NAD is a ubiquitous electron carrier essential for energy metabolism and the post translational modification of numerous regulatory proteins. Perturbation of NAD metabolism is considered detrimental to health, with NAD depletion commonly thought to promote aging. However, the extent to which cellular NAD concentration can be decreased without deleterious repercussions is unclear. We generated a mouse model where nicotinamide phosphoribosyltransferase (NAMPT)-mediated NAD+ biosynthesis is disrupted in adult skeletal muscle. The resulting 85% decrease in muscle NAD+ abundance was associated with preserved tissue integrity and functionality, as demonstrated by its unchanged morphology, contractility, and exercise tolerance. This lack of defects was corroborated by intact mitochondrial respiratory capacity and unaffected muscle transcriptomic and proteomic profiles. Furthermore, lifelong NAD depletion did not accelerate muscle aging or impair whole-body metabolism. Collectively, these findings indicate that NAD depletion does not contribute to age related declines in skeletal muscle function.
HostingRepository	PRIDE
AnnounceDate	2025-04-23
AnnouncementXML	Submission_2025-04-23_09:01:32.274.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Farina Leonie Schlabs
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-26 04:58:23	ID requested
⏵ 1	2025-04-23 09:01:32	announced

10.1016/J.CMET.2025.04.002;

submitter keyword: NAD metabolism

NAMPT

Skeletal muscle

Aging

exercise

mitochondrial supercomplexes

Jonas Thue Treebak
contact affiliation	Novo Nordisk Foundation Center for Basic Metabolic Reseach, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark
contact email	jttreebak@sund.ku.dk
lab head
Farina Leonie Schlabs
contact affiliation	Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
contact email	farina.schlabs@sund.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD061221

PRIDE project URI

• PRIDE
  1. PXD061221
     1. Label: PRIDE project
     2. Name: NAD Depletion in Skeletal Muscle does not Compromise Muscle Function or Accelerate Aging