PXD061221 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | NAD Depletion in Skeletal Muscle does not Compromise Muscle Function or Accelerate Aging |
Description | NAD is a ubiquitous electron carrier essential for energy metabolism and the post translational modification of numerous regulatory proteins. Perturbation of NAD metabolism is considered detrimental to health, with NAD depletion commonly thought to promote aging. However, the extent to which cellular NAD concentration can be decreased without deleterious repercussions is unclear. We generated a mouse model where nicotinamide phosphoribosyltransferase (NAMPT)-mediated NAD+ biosynthesis is disrupted in adult skeletal muscle. The resulting 85% decrease in muscle NAD+ abundance was associated with preserved tissue integrity and functionality, as demonstrated by its unchanged morphology, contractility, and exercise tolerance. This lack of defects was corroborated by intact mitochondrial respiratory capacity and unaffected muscle transcriptomic and proteomic profiles. Furthermore, lifelong NAD depletion did not accelerate muscle aging or impair whole-body metabolism. Collectively, these findings indicate that NAD depletion does not contribute to age related declines in skeletal muscle function. |
HostingRepository | PRIDE |
AnnounceDate | 2025-04-23 |
AnnouncementXML | Submission_2025-04-23_09:01:32.274.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Farina Leonie Schlabs |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | timsTOF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2025-02-26 04:58:23 | ID requested | |
⏵ 1 | 2025-04-23 09:01:32 | announced | |
Publication List
Keyword List
submitter keyword: NAD metabolism |
NAMPT |
Skeletal muscle |
Aging |
exercise |
mitochondrial supercomplexes |
Contact List
Jonas Thue Treebak |
contact affiliation | Novo Nordisk Foundation Center for Basic Metabolic Reseach, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark |
contact email | jttreebak@sund.ku.dk |
lab head | |
Farina Leonie Schlabs |
contact affiliation | Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark |
contact email | farina.schlabs@sund.ku.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD061221
- Label: PRIDE project
- Name: NAD Depletion in Skeletal Muscle does not Compromise Muscle Function or Accelerate Aging