PXD061087-1

PXD061087 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	An Unbiased Proteomic Platform for ATE1-based Arginylation Profiling-Ascend
Description	Protein arginylation is an essential posttranslational modification (PTM) catalyzed by arginyl-tRNA-protein transferase 1 (ATE1) in mammalian systems. Arginylation features a post-translational conjugation of an arginyl to a protein, making it extremely challenging to differentiate from translational arginine residues with the same mass in a protein sequence. Here we present a general ATE1-based arginylation profiling platform for the unbiased discovery of arginylation substrates and their precise modification sites. This method integrates isotopic arginine labeling into an ATE1 assay utilizing biological lysates (ex vivo) rather than live cells, thus eliminating translational bias derived from the ribosomal activity and enabling bona fide arginylation identification using isotopic features. The method has been successfully applied to an array of peptide, protein, cell, patient, and animal tissue samples using 20 ug sample input, with 235 unique arginylation sites revealed from human proteomes. Representative sites were validated and followed up for their biological functions. The developed platform is globally applicable to the aforementioned sample types and therefore paves the way for functional studies of this difficult-to-characterize protein modification.
HostingRepository	MassIVE
AnnounceDate	2025-06-18
AnnouncementXML	Submission_2025-06-18_21:48:56.629.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tom Lin
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	unknown modification
Instrument	Orbitrap Ascend

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-22 20:19:56	ID requested
⏵ 1	2025-06-18 21:48:57	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: arginylation, arginyltransferase, proteomics, DatasetType:Proteomics

submitter keyword: arginylation, arginyltransferase, proteomics, DatasetType:Proteomics

Contact List

Benjamin A. Garcia
contact affiliation	Washington University in St Louis
contact email	bagarcia@wustl.edu
lab head
Tom Lin
contact affiliation	Washington University in St Louis
contact email	zongtao@wustl.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097196/