PXD061086-1

PXD061086 is an original dataset announced via ProteomeXchange.

Title	Reprogramming SREBP1-dependent lipogenesis and inflammation in high-risk breast with licochalcone A: a novel path to cancer prevention
Description	This study investigates the effects of Licochalcone A (LicA) on protein stability in breast cancer cells using Proteome Integral Solubility Alterations (PISA) proteomics. We treated MCF-7 (ER+) and MDA-MB-231 (ER-) cells with LicA (10 µM) for 24 hours and subjected them to temperature-dependent protein solubility profiling. Thermal denaturation assays (40-65°C) followed by LC-MS/MS analysis with TMT labeling were performed to identify differentially stabilized and destabilized proteins. Our results indicate that LicA significantly destabilizes proteins involved in SREBP1-dependent lipogenesis, PI3K-AKT signaling, and NF-kB-mediated inflammation, while stabilizing pathways associated with antioxidant defense and metabolic reprogramming. These findings suggest a mechanism by which LicA alters the breast cancer proteome, leading to reduced tumor proliferation.
HostingRepository	PRIDE
AnnounceDate	2025-09-29
AnnouncementXML	Submission_2025-09-29_06:00:05.048.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shao Huan Samuel Weng
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-22 19:32:45	ID requested
⏵ 1	2025-09-29 06:00:05	announced

Dataset with its publication pending

submitter keyword: proteomics, licochalcone A, lipid metabolism, breast cancer prevention, LC-MS, SREBP, antiproliferation, Proteome Integral Solubility Alterations (PISA), PI3K-AKT pathway, metabolic reprogramming,Anti-inflammation

Atieh Hajirahimkhan
contact affiliation	Department of Surgery, Feinberg School of Medicine, Northwestern University, Chicago, IL 2Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, IL
contact email	atieh.hajirahimkhan@northwestern.edu
lab head
Shao Huan Samuel Weng
contact affiliation	Proteomics Platform, Office of Shared Research Facilities, University of Chicago
contact email	samuelweng@uchicago.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD061086

PRIDE project URI

• PRIDE
  1. PXD061086
     1. Label: PRIDE project
     2. Name: Reprogramming SREBP1-dependent lipogenesis and inflammation in high-risk breast with licochalcone A: a novel path to cancer prevention