PXD061033-1

PXD061033 is an original dataset announced via ProteomeXchange.

Title	Deciphering the impact of ectopic SNAI1 expression on renal tubular cell proteome and functions
Description	Snail1 is crucial in regulating epithelial mesenchymal transition (EMT), which leads to excessive extracellular matrix (ECM) production and organ fibrosis including the kidney. Elucidating the roles of snail1 in EMT and kidney fibrosis is thus vital for developing targeted therapies. This study investigated the alterations in the proteome of SNAI1-overexpressed proximal tubular epithelial cells over the vector-transfected cells by label-free quantitative proteomics. Of 670 identified proteins, 233 showed significant changes due to ectopic snail1 expression. Of these, immunoblotting confirmed the decreased HSP60 and HSP70 while increased DDX1. X2K Appyter predicted the top ten transcription factors as key upstream regulators of the altered proteome. KEGG enrichment analysis revealed that these altered proteins were primarily associated with translational regulation, ribosome, cell cycle regulation, and cellular senescence. GO enrichment showed that focal adhesion, the structure where cells maintain cell interior-ECM interactions, was markedly overrepresented. Accordingly, functional validations demonstrated that SNAI1-overexpressed cells displayed aberrant ribosome biogenesis, indicated by increasing nucleophosmin and the nucleolar organizer regions. SNAI1-overexpressed cells exhibited senescent phenotypes including cell enlargement and increased granularity, up-regulated senescent makers p21 and γH2AX, and enhanced MMP-9 secretion. Moreover, SNAI1-overexpressed cells up-regulated paxillin, a scaffold protein located at focal adhesions crucial for their dynamic regulation. These findings provide insights into the molecular processes governed by snail1 during renal tubular cells undergoing EMT.
HostingRepository	PRIDE
AnnounceDate	2026-04-27
AnnouncementXML	Submission_2026-04-26_16:19:39.665.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD061033
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Visith Thongboonkerd
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-20 20:22:53	ID requested
⏵ 1	2026-04-26 16:19:40	announced

10.1016/j.gendis.2025.101926;

Kanlaya R, Nonthawong K, Suntivichaya M, Yoodee S, Thongboonkerd V, gene on renal tubular cell proteome, nucleolar stress, ribosome biogenesis, senescence, DNA damage response, and focal adhesion dynamics. Genes Dis, 13(4):101926(2026) [pubmed]

10.6019/PXD061033;

submitter keyword: Renal fibrosis, Senescence, Proteomics, Snail1,Kidney

Prof. Visith Thongboonkerd
contact affiliation	Medical Proteomics Unit, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
contact email	vthongbo@yahoo.com
lab head
Visith Thongboonkerd
contact affiliation	Mahidol University
contact email	sirirajms@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061033
     1. Label: PRIDE project
     2. Name: Deciphering the impact of ectopic SNAI1 expression on renal tubular cell proteome and functions