PXD061020-1

PXD061020 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic analysis of fragments from non-bleached and bleached colonies of the hydrocoral Mille-pora complanata after the 2015–2016 ENSO event in the Mexican Caribbean
Description	In recent years, extensive mortality caused by global-scale bleaching events has led to the rapid degradation of reef structures, resulting in severe environmental consequences. The hydrocoral Millepora complanata (fire coral) plays a critical role in reef structure and relies on a symbiotic relationship with Symbiodiniaceae. Environmental stressors derived from climate change, such as UV radiation and elevated temperatures disrupt this symbiosis, leading to bleaching and threatening reef survival. Despite its importance in reef building, the molecular response of this cnidarian to thermal stress have been poorly explored. For this reason, the objective of this study is to investigate the proteomic response of M. complanata to bleaching during the 2015–2016 El Niño event. Fragments from non-bleached and bleached colonies of the hydrocoral M. complanata were collected from a coral reef in the Mexican Caribbean, and proteomic extracts were analyzed using nano-liquid chromatography–tandem mass spectrometry (nano-LC-MS/MS). Uni- and multivariate analyses were applied to identify significant differences in protein abundance. A total of 52 proteins showed differential abundance, including 24 that showed increased expression and 28 whose expression decreased in bleached fragments. Differentially abundant proteins were associated with amino acid biosynthesis, carbohydrate metabolism, cytoskeleton organization, DNA repair, extracellular matrix composition, redox homeostasis, and protein modification. These findings indicate that heat stress triggers molecular responses involving protein refolding, enhanced vesicle transport, cytoskeletal reorganization, and adjustments in redox activity, thereby contributing to a better understanding of the molecular mechanisms underlying bleaching in reef-building hydrocorals.
HostingRepository	PRIDE
AnnounceDate	2025-09-15
AnnouncementXML	Submission_2025-09-14_16:36:48.540.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Esteban de Jesús Alcantar-Orozco
SpeciesList	scientific name: Millepora complanata; NCBI TaxID: 544496; scientific name: Cnidaria; NCBI TaxID: NCBITaxon:6073;
ModificationList	2-pyrrolidone-5-carboxylic acid (Glu); 2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-20 13:16:13	ID requested
⏵ 1	2025-09-14 16:36:50	announced

10.3390/biology14081042;

Alcantar-Orozco EJ, Hern, á, ndez-Eliz, á, rraga VH, Vega-Tamayo JE, Ibarra-Alvarado C, Caballero-P, é, rez J, Rodr, í, guez de San Miguel E, Rojas-Molina A, Reveals Stress Response Signatures Following the 2015-2016 ENSO Event in the Mexican Caribbean. Biology (Basel), 14(8):(2025) [pubmed]

submitter keyword: proteomic analysis, reef building cnidarians,Millepora complanata, hydrocorals

Alejandra Rojas
contact affiliation	Universidad Autónoma de Querétaro
contact email	Rojasa@uaq.mx
lab head
Esteban de Jesús Alcantar-Orozco
contact affiliation	Autonomous University of Queretaro
contact email	estebanalcantar@hotmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061020
     1. Label: PRIDE project
     2. Name: Comparative proteomic analysis of fragments from non-bleached and bleached colonies of the hydrocoral Mille-pora complanata after the 2015–2016 ENSO event in the Mexican Caribbean