PXD061017-1

PXD061017 is an original dataset announced via ProteomeXchange.

Title	Dynamic subcellular proteomics identifies novel regulators of adipocyte insulin action
Description	Insulin acts on adipocytes to control whole-body glucose and lipid metabolism by increasing glucose uptake and inhibiting lipolysis. These processes are dependent on changes in protein localisation (e.g GLUT4 translocation to the plasma membrane (PM)). However, our knowledge of spatial proteomic changes in response to insulin is limited. Here, we use subcellular proteomics to map insulin-stimulated protein relocalisation at a cell-wide scale. Approximately 10% of proteins identified exhibited altered subcellular localisation in response to insulin. Since the PM was a major site of insulin-responsive proteome remodelling, we performed additional PM proteome profiling to quantify insulin-responsive changes at the cell surface. These orthologous proteomic approaches revealed insulin-stimulated redistribution of C3ORF18 to the PM. Studies in adipocytes depleted of C3ORF18 suggest this protein is required for maximal insulin signalling. Overall, our data provide an accessible resource of insulin-regulated changes in protein localisation and impetus for further work on how changes in protein localisation contribute to the cellular insulin response.
HostingRepository	PRIDE
AnnounceDate	2026-02-06
AnnouncementXML	Submission_2026-02-06_10:39:32.587.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olivia Conway
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-20 12:54:23	ID requested
⏵ 1	2026-02-06 10:39:33	announced

Dataset with its publication pending

submitter keyword: Mouse, adipocyte

Daniel Fazakerley
contact affiliation	Institute of Metabolic Science, University of Cambridge, UK
contact email	djf72@cam.ac.uk
lab head
Olivia Conway
contact affiliation	University of Cambridge
contact email	oc312@cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061017
     1. Label: PRIDE project
     2. Name: Dynamic subcellular proteomics identifies novel regulators of adipocyte insulin action