PXD061003-1

PXD061003 is an original dataset announced via ProteomeXchange.

Title	Formation of a membraneless compartment regulates bacterial virulence
Description	The RNA-binding protein CsrA regulates the expression of hundreds of genes in several bacterial species, thus controlling virulence and other processes. However, the outcome of the CsrA-mRNA interactions is modulated by competing small RNAs and other factors through mechanisms that are only partially understood. Here, we show that CsrA accumulates in a dynamic membraneless compartment in cells of E. coli and other pathogenic species. In addition to CsrA, the compartment contains components of the RNA-degrading complex (degradosome), regulatory small RNAs, and selected mRNAs. Formation of the compartment is associated with a switch between promoting and repressing virulence gene expression by CsrA. We suggest that similar CsrA switches may be widespread in diverse bacteria.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_07:27:48.030.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Keren Bendalak
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-20 02:58:02	ID requested
⏵ 1	2025-05-07 07:27:48	announced

Aroeti L, Elbaz N, Faigenbaum-Romm R, Yakovian O, Altuvia Y, Argaman L, Katsowich N, Bejerano-Sagie M, Ravins M, Margalit H, Ben-Yehuda S, Rosenshine I, Formation of a membraneless compartment regulates bacterial virulence. Nat Commun, 16(1):3834(2025) [pubmed]

10.1038/s41467-025-58829-9;

submitter keyword: membraneless compartment, CsrB, type III secretion system, virulence regulation,enteropathogenic bacteria, host-pathogen interaction, enteropathogenic E. coli, sRNA, CsrA, post-transcriptional regulation

Prof. Ilan Rosenshine
contact affiliation	Hebrew university, Jerusalem
contact email	ilanr@ekmd.huji.ac.il
lab head
Keren Bendalak
contact affiliation	Technion
contact email	kerenb@technion.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD061003

PRIDE project URI

• PRIDE
  1. PXD061003
     1. Label: PRIDE project
     2. Name: Formation of a membraneless compartment regulates bacterial virulence