PXD060990-1

PXD060990 is an original dataset announced via ProteomeXchange.

Title	Vitamin C potentiates certain protein synthesis inhibiting antibiotics such as sulfamethoxazole by disrupting copper homeostasis
Description	In this project, we initially discovered the significant synergistic effects of vitamin C (Vit C) and Sulfamethoxazole (SMX) on various strains in both laboratory and clinical settings. To search for potential clues at the proteomic level for the synergistic property of Vit C+SMX observed in vitro, we quantitatively monitored changes in the proteome of cells treated with Vit C (1 mM), SMX (10 µg/ml), a mixture of both drugs at their respective concentrations, and a control group (no drug treatment). Mass spectrometry data were collected in Data Independent Acquisition (DIA) mode using a Q-Exactive HF X (Thermo Fisher Scientific, San Jose, CA) instrument for 36 samples (consisting of three biological replicates each of the above four groups of samples harvested after 12, 24, and 36 hours of treatment, respectively), quantifying a total of 37, 363 peptides and 2,685 proteins. Quantification of peptides and proteins was performed using MSstats software packages. After conducting enrichment analyses and functional annotation for proteins that were differentially expressed exclusively in the vc+smx group, we identified a consistent up-regulation of numerous proteins associated with copper homeostasis and redox stress. Coupled with the validation of a series of in vivo and ex vivo experiments, we elucidated the molecular mechanisms underlying the bactericidal effect of Vit C potentiation of SMX.
HostingRepository	iProX
AnnounceDate	2025-02-20
AnnouncementXML	Submission_2025-10-20_01:49:02.912.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mi Wen
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-19 22:38:06	ID requested
⏵ 1	2025-10-20 01:49:03	announced

Dataset with its publication pending

submitter keyword: Vitamin C, Sulfamethoxazole, E.coli, Proteomics analysis, DDA, DIA, copper homeostasis

Jiaoyu Deng
contact affiliation	Wuhan Institute of Virology, CAS
contact email	dengjy@wh.iov.cn
lab head
Mi Wen
contact affiliation	Wuhan Institute of Virology, CAS
contact email	2586890379@qq.com
dataset submitter

iProX dataset URI