PXD060986-1

PXD060986 is an original dataset announced via ProteomeXchange.

Title	In-Situ Ammonia Recovery in Anaerobic digestion via Gas Permeable Membranes: Reshaping Dominant Methanogens under Ammonia Stress through Divergent Insights from Microbiomics, Metagenomics, and Metaproteomics
Description	Mechanistically, this study reconstructs the dominant methanogens under ammonia stress through insights from microbiomics, metagenomics, and metaproteomics. The research identifies Methanobacterium as a "pseudo dominant genus" under ammonia stress—large in population but low in contribution. The significant downregulation of electron transfer efficiency and methyl-reducing protein expression in Methanobacterium may be key factors limiting its activity. Conversely, the study confirms Methanothrix as the "true dominant genus" under ammonia inhibition—reduced in population but undiminished in contribution. Methanothrix expresses all proteins required for acetoclastic and hydrogenotrophic methanogenesis and upregulates functional proteins related to post-translational modifications, tRNA activation, and initiation complex formation during enzyme synthesis. Finally, metaproteomics unveiled the presence of "exceptionally active genera" with remarkably low community abundance—specifically, Methanolinea, Methanospirillum, and unclassified_f__Methanoregulaceae. Their protein expression levels in the methanogenesis pathway surpassed those of Methanobacterium, establishing them as the primary drivers of hydrogenotrophic methanogenesis.
HostingRepository	iProX
AnnounceDate	2025-02-19
AnnouncementXML	Submission_2025-07-31_19:27:58.518.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Linjun Wu
SpeciesList	scientific name: anaerobic bacterium foal30; NCBI TaxID: 1141423;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-19 19:18:38	ID requested
⏵ 1	2025-07-31 19:27:58	announced

Dataset with its publication pending

submitter keyword: Anaerobic digestion, Ammonia Recovery, Gas Permeable Membranes, Microbiomics, Metagenomics, and Metaproteomics.

Zhiqiang Shen
contact affiliation	Research Center of Environmental Pollution Control Engineering Technology, Chinese Research Academy of Environmental Sciences
contact email	shenzq@craes.org.cn
lab head
Linjun Wu
contact affiliation	Research Center of Environmental Pollution Control Engineering Technology, Chinese Research Academy of Environmental Sciences
contact email	346189594@qq.com
dataset submitter

iProX dataset URI