PXD060980-1

PXD060980 is an original dataset announced via ProteomeXchange.

Title	Chemoproteomic Profiling of Reactive Cysteines in Response to Oxidative Stress Induced by 6-Hydroxydopamine
Description	Oxidative stress is a well-known driver of neurodegeneration, yet it remains poorly understood how the global cysteine (Cys) proteome is remodeled in such conditions. Proteins with aberrantly modified Cys in response to oxidative stress can induce and exacerbate neurodegeneration. In this study, we induced oxidative stress by treating SH-SY5Y neuronal cells with the neurotoxin 6-hydroxydopamine (6-OHDA). To identify proteins with Cys that had altered oxidation or post-translational modification statuses, we utilized a desthiobiotin iodoacetamide (DBIA) probe, which selectively labels Cys with unmodified and preserved thiols. We identified numerous proteins that exhibited reduced or enhanced Cys reactivity to DBIA in response to 6-OHDA-induced oxidative stress, many of which are critically involved in biological processes linked to cell stress responses (e.g., mitochondrial oxidative stress and apoptosis). Our study defines the remodeling of the Cys proteome and suggests potential Cys-mediated regulatory mechanisms under 6-OHDA-induced oxidative stress conditions. In the context of neurodegeneration and under these conditions, our findings provide a valuable resource for further exploration of the Cys proteome.
HostingRepository	PRIDE
AnnounceDate	2025-10-20
AnnouncementXML	Submission_2025-10-19_16:25:16.545.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rayan Murtada
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-19 14:15:00	ID requested
⏵ 1	2025-10-19 16:25:17	announced

10.1002/pmic.70050;

Murtada R, Kim C, Wang XD, Yang Y, Yu Y, Chemoproteomic Profiling of Reactive Cysteines in Response to Oxidative Stress Induced by 6-Hydroxydopamine. Proteomics, ():e70050(2025) [pubmed]

submitter keyword: Quantitative proteomics, cell stress response, SH-SY5Y, oxidative stress, neurodegeneration, neurodegenerative signaling, 6-hydroxydopamine, cysteine proteomics

Yonghao Yu
contact affiliation	Department of Molecular Pharmacology and Therapeutics, Columbia University Irving Medical Center, New York, NY, 10032
contact email	yy3213@cumc.columbia.edu
lab head
Rayan Murtada
contact affiliation	Columbia University Irving Medical Center
contact email	rm4066@cumc.columbia.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060980
     1. Label: PRIDE project
     2. Name: Chemoproteomic Profiling of Reactive Cysteines in Response to Oxidative Stress Induced by 6-Hydroxydopamine