PXD060979-1

PXD060979 is an original dataset announced via ProteomeXchange.

Title	The ribonuclease DISL-2 mediates piRNA degradation
Description	The PIWI/piRNA pathway acts as a conserved RNA-directed immune mechanism that suppresses transposons and promotes fertility. The biogenesis of piRNAs has been extensively investigated since their discovery, yet the mechanisms governing piRNA degradation remain poorly understood. Here, we systematically measure the half-lives of thousands of C. elegans piRNAs, revealing a broad range from less than 1 hour to over 20 hours. Notably, piRNAs with 3' terminal uridines (U) degrade faster than those with 3' guanines or cytosines, suggesting that 3' uridines induce nucleolytic degradation. We identify DISL-2, a 3' to 5' exoribonuclease, as a key enzyme responsible for degrading piRNAs with templated 3' uridines as well as non-templated 3' uridines added by Poly(U) polymerase PUP-1. Furthermore, DISL-2 acts as a quality control factor to target piRNAs that are improperly processed at their 3' ends. Together, these findings advance our understanding of piRNA homeostasis by determining piRNA half-lives and identifying factors involved piRNA decay.
HostingRepository	PRIDE
AnnounceDate	2025-09-07
AnnouncementXML	Submission_2025-09-07_09:01:45.631.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD060979
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	WEN TANG
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-19 13:01:05	ID requested
⏵ 1	2025-09-07 09:01:46	announced

10.6019/PXD060979;

submitter keyword: piRNA degradation, ribonuclease,PIWI

Wen Tang
contact affiliation	Department of Biological Chemistry and Pharmacology, The Ohio State University, Columbus, OH 43210, USA. Center for RNA Biology, The Ohio State University, Columbus, OH 43210, USA.
contact email	tang.542@osu.edu
lab head
WEN TANG
contact affiliation	Ohio State University
contact email	tang.542@osu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060979
     1. Label: PRIDE project
     2. Name: The ribonuclease DISL-2 mediates piRNA degradation