PXD060971-1

PXD060971 is an original dataset announced via ProteomeXchange.

Title	ANXA11 biomolecular condensates facilitate protein-lipid phase coupling on lysosomal membranes
Description	Phase transitions of cellular proteins and lipids play a key role in governing the organisation and coordination of intracellular biology. Recent work has raised the intriguing prospect that phase transitions in proteins and lipids can be co-regulated. Here we investigate this possibility in the ribonucleoprotein (RNP) granule-ANXA11-lysosome ensemble, where ANXA11 tethers RNP granules to lysosomal membranes to enable their co-trafficking. We show that changes to the protein phase state within this system, driven by the low complexity ANXA11 N-terminus, induces a coupled phase state change in the lipids of the underlying membrane. We identify the ANXA11 interacting proteins ALG2 and CALC as potent regulators of ANXA11-based phase coupling and demonstrate their influence on the nanomechanical properties of the ANXA11-lysosome ensemble and its capacity to engage RNP granules. The phenomenon of protein-lipid phase coupling we observe within this system serves as a potential regulatory mechanism in RNA trafficking and offers an important template to understand other examples across the cell whereby biomolecular condensates closely juxtapose organellar membranes.
HostingRepository	PRIDE
AnnounceDate	2025-02-19
AnnouncementXML	Submission_2025-02-19_09:50:43.276.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Robin Antrobus
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-19 09:41:31	ID requested
⏵ 1	2025-02-19 09:50:43	announced

Dataset with its publication pending

submitter keyword: LC-MSMS, RNP granules,ANXA11

Dr Jonathon Nixon-Abell
contact affiliation	Cambridge Institute for Medical Research, Department of Clinical Neurosciences, Clinical School, University of Cambridge, Cambridge, CB2 0XY, UK
contact email	jjn36@cam.ac.uk
lab head
Robin Antrobus
contact affiliation	CIMR medicine
contact email	pra29@cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060971
     1. Label: PRIDE project
     2. Name: ANXA11 biomolecular condensates facilitate protein-lipid phase coupling on lysosomal membranes