PXD060953 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Transcriptome-Proteome analysis of human naive and memory B cell subsets reveal isotype and subclass-specific phenotypes |
| Description | Antibodies produced by B cells aid in recognition and clearance of pathogens and is the cornerstone of vaccination strategies. Humans produce nine different antibody isotypes and their effector functions differ according to the type of antigen and route of exposure. Phenotypic variation between isotype-swithched B cell subsets is expected but not studied in detail. To obtain a molecular definition of isotype-defined B cell identity, we performed proteomics and transcriptomics on isotype-defined populations of human naive and memory B cells (MBCs): CD27-IgM+IgD+, CD27+CD38lo/-IgM+IgD+, CD27+CD38lo/-IgM+IgD-, and IgA1, IgA2, IgG1, IgG2, IgG3, and IgG4 MBCs (CD27+CD38lo/-Ig+). Combined proteome and transcriptome analysis revealed that mRNA and protein expression profiles segregate separate isotype-defined B cell subsets according to their differentiation status. mRNA and protein expression levels correlated reasonably well for many genes. IgG4+ MBCs were most distinct from naive B cells. Besides a distinct expression profile of cytokine and Fc receptors, we identified a high expression of IgE-coding mRNA in IgG4-switched B cells. SDR16C5 was identified as uniquely upregulated in IgG4-switched B cells. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-12 |
| AnnouncementXML | Submission_2026-03-12_16:07:41.997.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Arie Hoogendijk |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-02-19 05:09:29 | ID requested | |
| ⏵ 1 | 2026-03-12 16:07:42 | announced | |
Publication List
Keyword List
| submitter keyword: Isotypes, Transcriptomics,B cells, Proteomics, IgG4 |
Contact List
| Maartje van den Biggelaar, PhD |
|---|
| contact affiliation | Sanquin Research, NL |
| contact email | m.vandenbiggelaar@sanquin.nl |
| lab head | |
| Arie Hoogendijk |
|---|
| contact affiliation | Sanquin Research, Amsterdam |
| contact email | a.hoogendijk@sanquin.nl |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD060953
- Label: PRIDE project
- Name: Transcriptome-Proteome analysis of human naive and memory B cell subsets reveal isotype and subclass-specific phenotypes
