PXD060948-1

PXD060948 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Elucidation of B-cell specific drug immunogenicity liabilities via a novel ex vivo assay
Description	The appended raw files, csv files and other documents were deposited in the public domain in support for the publication "Elucidation of B-cell specific drug immunogenicity liabilities via a novel ex vivo assay" by Cary M Looney, Axel Ducret, Guido Steiner, Karen Dernick, Katharina Hartman, Michel Siegel, Timothy Hickling, Alex Odermatt, Celine Marban-Doran. The abstract is as follows: The advent of large molecule therapeutics has revolutionized treatment options for previously unmet medical needs. This advent has also led to an increased impact of immunogenicity on drug efficacy and safety. In order to maximize the potential of large molecule therapeutics, immunogenicity-related liabilities must be identified as early in development as possible, using an integrated risk assessment that takes into account the various cell types and processes involved. Here, we describe the development of an ex vivo B-cell immunogenicity assay, to capture a key component of the immune response that has been missing from previously published ex vivo immunogenicity assays. Plasmablasts/plasma cells were preferentially expanded in this assay, a subset of which were drug-specific and presented drug-specific peptides on MHC Class II. This assay represents an important tool in the immunogenicity risk assessment toolkit, to allow liabilities to be identified and mitigated early in the drug development process.
HostingRepository	MassIVE
AnnounceDate	2025-07-08
AnnouncementXML	Submission_2025-07-08_06:20:11.997.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Axel Ducret
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Deamidated; Gln->pyro-Glu; L-methionine sulfoxide
Instrument	timsTOF Pro 2

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-19 00:49:06	ID requested
⏵ 1	2025-07-08 06:20:12	announced

Publication List

Looney CM, Ducret A, Steiner G, Dernick K, Hartman K, Siegel M, Hickling T, Odermatt A, Marban-Doran C, assay. Front Immunol, 16():1589483(2025) [pubmed]

Looney CM, Ducret A, Steiner G, Dernick K, Hartman K, Siegel M, Hickling T, Odermatt A, Marban-Doran C, assay. Front Immunol, 16():1589483(2025) [pubmed]

Keyword List

submitter keyword: immunogenicity, in vitro B cell assay, anti-drug antibodies, MAPPs, DatasetType:Proteomics

submitter keyword: immunogenicity, in vitro B cell assay, anti-drug antibodies, MAPPs, DatasetType:Proteomics

Contact List

Axel Ducret
contact affiliation	Roche Innovation Center Basel
contact email	axel.ducret@roche.com
lab head
Axel Ducret
contact affiliation	F. Hoffmann-La Roche Ltd
contact email	axel.ducret@roche.com
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097151/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097151/