PXD060946-1

PXD060946 is an original dataset announced via ProteomeXchange.

Title	Cyanobacterial Argonautes and Cas4 family nucleases cooperate to interfere with invading DNA
Description	Prokaryotic Argonaute proteins (pAgos) from the long-A clade are stand-alone immune systems that use small interfering (si)DNA guides to recognize and cleave invading plasmid and virus DNA. Certain long-A pAgos are co-encoded with accessory proteins with unknown functions. Here, we show that cyanobacterial long-A pAgos act in conjunction with Argonaute-associated Cas4 family enzyme 1 (ACE1). Structural and biochemical analyses reveal that ACE1-associated pAgos mediate siDNA-guided DNA interference, akin to stand-alone pAgos. ACE1 is structurally homologous to the nuclease domain of bacterial DNA repair complexes and acts as a single-stranded DNA endonuclease that processes siDNA guides. pAgo and ACE1 form a heterodimeric APACE1 complex, which modulates ACE1 activity. While ACE1-associated pAgos alone interfere with plasmids and bacteriophages, plasmid interference is boosted when pAgo and ACE1 are co-expressed. Our study reveals that pAgo-mediated immunity is enhanced by accessory proteins and broadens our mechanistic understanding of how pAgo systems interfere with invading DNA.
HostingRepository	PRIDE
AnnounceDate	2025-04-15
AnnouncementXML	Submission_2025-04-15_00:19:59.312.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD060946
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Mark Roosjen
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-19 00:08:58	ID requested
⏵ 1	2025-04-15 00:19:59	announced

10.6019/PXD060946;

submitter keyword: MaAGO, MaACE1,APACE

Daan Swarts
contact affiliation	Principle investigator
contact email	daan.swarts@wur.nl
lab head
Mark Roosjen
contact affiliation	Wageningen University and Research
contact email	mark.roosjen@wur.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060946
     1. Label: PRIDE project
     2. Name: Cyanobacterial Argonautes and Cas4 family nucleases cooperate to interfere with invading DNA