PXD060935-1

PXD060935 is an original dataset announced via ProteomeXchange.

Title	Extracellular vesicles proteomic profile in anti-TNF immunosuppressed mice: insights into protein dysregulation during Leishmania infantum infection
Description	Visceral leishmaniasis (VL) is most frequent in immunosuppressed patients, especially among those receiving immunosuppressive therapy for autoimmune diseases, being TNF antagonist (anti-TNF) one of the main immunosuppressants used. Under these conditions, VL is most severe, with lower response to traditional leishmanicidal treatments such as antimonials (Sb) and a higher risk of relapse. Extracellular vesicles (EVs) are a great source of biological molecules, including proteins, capable of modulating immune-mediated cells and virulence factors. To identify proteins involved in the clinic of VL under anti-TNF immunosuppression, the proteomic profile of EVs from the serum of BALB/c mice was analysed using a label-free quantification (LFQ) proteomics approach. In infected mice with Leishmania infantum a comparison was made between immunosuppression with anti-TNF at clinical doses and non-immunosuppressed conditions, before and after treatment with Sb. Proteomics analysis revealed 271 dysregulated proteins in the pre-treatment groups, the majority of which were downregulated in the anti-TNF samples. On the other hand, 216 proteins were identified in the treated groups, being most of them enriched in the anti-TNF treated (anti-TNF+Sb) samples. The gene ontology (GO) analysis of these proteins revealed altered pathways related to the immune system, liver regeneration, or ion transport. Our results show dysregulated proteins associated with anti-TNF immunosuppression, which could have an impact on the greatest severity of the VL infection experienced in this situation. This study is an important step in the understanding of the clinical management of these VL pharmacologically immunosuppressed cases.
HostingRepository	PRIDE
AnnounceDate	2025-07-22
AnnouncementXML	Submission_2025-07-22_04:48:49.787.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ana Montero Calle
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-18 16:37:10	ID requested
⏵ 1	2025-07-22 04:48:50	announced

10.3389/FIMMU.2025.1634080;

submitter keyword: antimonials, visceral leishmaniasis, immunosuppression,Extracelullar vesicles, quantitative proteomics, biomarkers, TNF antagonist

Rodrigo Barderas
contact affiliation	Chronic Disease Program (UFIEC), Instituto de Salud Carlos III, Majadahonda, Madrid, Spain
contact email	r.barderasm@isciii.es
lab head
Ana Montero Calle
contact affiliation	Instituto de Salud Carlos III
contact email	ana.monteroc@isciii.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060935
     1. Label: PRIDE project
     2. Name: Extracellular vesicles proteomic profile in anti-TNF immunosuppressed mice: insights into protein dysregulation during Leishmania infantum infection