PXD060927-1

PXD060927 is an original dataset announced via ProteomeXchange.

Title	Development of a General Purpose Targeted LC-MS Method for Accurate Quantification of the SARS-CoV-2 Spike protein expression
Description	The COVID-19 pandemic has catalyzed interest in immuno-multiple reaction monitoring (immuno-MRM) methods, with the detection of peptides unique to the nucleocapsid protein in nasopharyngeal swabs. While current applications predominantly focus on disease biomarkers, the pandemic has unveiled new opportunities, namely for the quantification of antigen expression following mRNA vaccination. Here, we present an optimized immuno-MRM method for quantifying SARS-CoV-2 spike protein fusion peptide, SFIEDLLFNK, for several practical applications. The method is versatile, applicable to multiple biological matrices, including plasma, and can be extended to nasopharyngeal swabs. It also offers a high-precision tool for assessing protein expression following plasmid and mRNA transfection. Moreover, in parallel to enabling accurate antigen quantification, the flow-through can be used to determine the proteome profile of the infected cells, providing insights into the intracellular immune response. This dual capability supports the rapid optimization of mRNA vaccines, thereby driving advancements in vaccine development strategies.
HostingRepository	PanoramaPublic
AnnounceDate	2025-09-25
AnnouncementXML	Submission_2025-09-25_21:27:48.032.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Bart Van Puyvelde
SpeciesList	scientific name: Severe acute respiratory syndrome coronavirus 2; NCBI TaxID: 2697049; scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Oxidation; Label:15N(1); Label:15N(2); Label:15N(4)
Instrument	ZenoTOF 7600; Xevo TQ-XS; TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-18 11:25:51	ID requested
⏵ 1	2025-09-25 21:27:48	announced

no publication

submitter keyword: Mass Spectrometry, SARS-CoV-2, immuno-MRM, RNA expression, SPIKE_SARS2

Maarten Dhaenens
contact affiliation	ProGenTomics, Laboratory of Pharmaceutical Biotechnology, Ghent University
contact email	maarten.dhaenens@ugent.be
lab head
Bart Van Puyvelde
contact affiliation	ProGenTomics, Laboratory of Pharmaceutical Biotechnology, Ghent University
contact email	bart.vanpuyvelde@ugent.be
dataset submitter

Panorama Public dataset URI