PXD060917-1

PXD060917 is an original dataset announced via ProteomeXchange.

Title	X-Ray Exposure Induces Structural Changes in Human Breast Proteins
Description	During radiotherapy, X-rays can deliver significant doses of ionising radiation to both cancerous and surrounding healthy tissues, often leading to undesirable side effects that compromise patient outcomes. While the cellular effects of such therapeutic x-ray exposures are well studied, the impact on extracellular matrix (ECM) proteins, which are critical to tissue integrity, remains poorly understood. This study characterises the response of breast proteins, including the major ECM components collagen I and fibronectin, to therapeutic X-rays doses of 50Gy (as employed in breast radiotherapy) and 100Gy, using a combination of gel electrophoresis, biochemical assays, and mass spectrometry-based peptide location fingerprinting (PLF) analysis. In purified protein solutions, X-ray exposure led to fragmentation of constituent α chains of collagen I, and in fibronectin localised structural modifications (as detected by LC-MS/MS peptide location fingerprinting [PLF]) which increased its binding affinity for collagen I. In complex environments, such as newly synthesised fibroblast-derived ECM (fECM) and mature ex vivo breast tissue, therapeutic x-rays induced proteolytic changes in, not just collagen I and fibronectin, but also key basement membrane proteins, including collagen IV, laminin, nidogen-1, and perlecan. Intracellular proteins associated with gene expression (RPS3, MeCP2), cytoskeleton (moesin, plectin), and the endoplasmic reticulum (calnexin) were also found to be structurally compromised. These structural changes may impair the ECM integrity and alter cell-ECM interactions, with potential implications for tissue stiffening, fibrosis, and impaired wound healing in irradiated tissues.
HostingRepository	PRIDE
AnnounceDate	2025-06-28
AnnouncementXML	Submission_2025-06-27_21:21:18.743.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD060917
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ren Jie Tuieng
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-18 06:40:29	ID requested
⏵ 1	2025-06-27 21:21:19	announced

10.3390/IJMS26125696;

10.6019/PXD060917;

submitter keyword: radiation, fibronectin, therapy,human, ecm

Michael Sherratt
contact affiliation	Cell matrix biology and regenerative medicine, School of biological sciences, University of manchester
contact email	michael.j.sherratt@manchester.ac.uk
lab head
Ren Jie Tuieng
contact affiliation	National university of Singapore
contact email	snrrjt@nus.edu.sg
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060917
     1. Label: PRIDE project
     2. Name: X-Ray Exposure Induces Structural Changes in Human Breast Proteins