PXD060890-1

PXD060890 is an original dataset announced via ProteomeXchange.

Title	Dysregulated pathways and potential biomarkers in dermatomyositis plasma-derived extracellular vesicles
Description	Extracellular vesicles (EVs) have been implicated in dermatomyositis pathogenesis, but their protein content is not well characterized. We collected EVs from plasma, isolated them using sequential ultracentrifugation and size-exclusion chromatography, and analyzed their content by mass spectrometry. Protein biomarkers that distinguish patients with DM from controls were assessed using the random forest algorithm. Out of these, select proteins were identified as highly potential disease biomarkers, and their abundance was confirmed in a separate cohort using ELISA. Thirty-five proteins were differentially expressed and 67 were uniquely detected in the patient cohort. The following proteins displayed the most significant differential expression and were validated in a separate cohort of patients: The antioxidant enzyme glutathione peroxidase 3 (GPX3), and two complement proteins, Ficolin-2 (FCN2) and SERPING1 were less abundant in patients' EVs. Surfactant protein B (SFTPB) was expressed almost exclusively in patients with lung disease, suggesting that it may be a marker for pulmonary involvement. In summary, we identified GPX3, FCN2 and SERPING1 as biomarkers in DM. Our data underline the importance of oxidative stress and complement regulation in DM. SFTPB is a potential biomarker for pulmonary involvement.
HostingRepository	PRIDE
AnnounceDate	2025-07-10
AnnouncementXML	Submission_2025-07-10_11:08:48.705.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hossein Fazelinia
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-17 13:22:35	ID requested
⏵ 1	2025-07-10 11:08:49	announced

Dataset with its publication pending

submitter keyword: Extracellular vesicles, Plasma, dermatomyositis pathogenesis, oxidative stress

Victoria P. Werth, MD
contact affiliation	Division of Dermatology Veterans Administration Hospital University and Woodland Avenues Philadelphia, PA 19104 Office: 215-823-4208
contact email	Victoria.Werth@pennmedicine.upenn.edu
lab head
Hossein Fazelinia
contact affiliation	Children's Hospital of Philadelphia
contact email	fazeliniah@email.chop.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD060890

PRIDE project URI

• PRIDE
  1. PXD060890
     1. Label: PRIDE project
     2. Name: Dysregulated pathways and potential biomarkers in dermatomyositis plasma-derived extracellular vesicles