PXD060878-1

PXD060878 is an original dataset announced via ProteomeXchange.

Title	Mapping the intracellular HMGB1 interactome and alterations induced by Toll-like receptor 4 activation
Description	THP1 cells were transduced with MycBioID2-HMGB1 and thereafter cultured in SILAC media for 1 week. Cells were treated with doxycycline (2 ug/ml, 48h) to induce gene expression. Thereafter, doxycycline was removed and heavy labelled cells were stimulated with LPS (100 ng/ml) for 1 hour before addition of biotin (24 hours). Light cells were used as control. Biotinylated proteins were extracted and identified with LC-MS/MS
HostingRepository	PRIDE
AnnounceDate	2025-11-11
AnnouncementXML	Submission_2025-11-10_22:59:51.583.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rebecka Heinbäck
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	6x(13)C labeled residue; monohydroxylated residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-17 07:32:34	ID requested
⏵ 1	2025-11-10 22:59:52	announced

10.1016/j.jbc.2025.110866;

Heinb, ä, ck R, van der Zijde M, Tsang CH, Tr, š, eli, č T, Espinosa A, Aulin C, Erlandsson Harris H, Mapping the intracellular HMGB1 interactome and alterations induced by Toll-like receptor 4 activation. J Biol Chem, 301(12):110866(2025) [pubmed]

submitter keyword: None

Helena Erlandsson
contact affiliation	Division of Rheumatology, Department of Medicine Solna, Karolinska Institutet, and Center for Molecular Medicine, Karolinska University Hospital (,SE-171 76 Stockholm, Sweden). Broegelmann Research Laboratory, Department of Clinical Science, University of Bergen, Norway.
contact email	helena.harris@ki.se
lab head
Rebecka Heinbäck
contact affiliation	Karolinska Institute, Sweden
contact email	rebecka.heinback@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD060878

PRIDE project URI

• PRIDE
  1. PXD060878
     1. Label: PRIDE project
     2. Name: Mapping the intracellular HMGB1 interactome and alterations induced by Toll-like receptor 4 activation