PXD060857-1
PXD060857 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Efficient fabrication of human corneal stromal cell spheroids and promoting cell stemness based on 3D-printed derived PDMS microwell platform |
| Description | Here, we introduce a novel three-dimensional (3D) cell culture system based on a pol-ydimethylsiloxane (PDMS) microwell platform composed of many V-bottom microcavities for the generation of human corneal stromal spheroids. We isolated hCSCs from SMILE-derived lenticules and maintained their physiological phenotype by culturing them in a medium supplemented with human corneal stromal extract (hCSE). Utilizing a PDMS microwell platform fabricated through 3D printing technology, we successfully generated 3D corneal stromal cell spheroids (3D-CSC) with uniform size and stable structure, exhibiting increased expression of pluripotency factors, including OCT4, NANOG, SOX2, KLF4, and PAX6. Furthermore, the iPS supernatant of E8-conditioned medium (E8-CM) significantly enhanced the stemness properties of these cells. RNA sequencing and proteomics analyses revealed that 3D-CSCs exhibited superior prolif-eration, differentiation, cell adhesion, migration, and neurogenesis compared to tradi-tional monolayer cultures, underscoring the role of biophysical cues in promoting hCSCs stemness. |
| HostingRepository | iProX |
| AnnounceDate | 2025-02-15 |
| AnnouncementXML | Submission_2025-02-16_19:20:11.104.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yuexi Chen |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-02-16 19:19:14 | ID requested | |
| ⏵ 1 | 2025-02-16 19:20:11 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: human corneal stromal cells, SMILE, Three-dimensional spheroid culture, PDMS, stem cells, stemness, pluripotency |
Contact List
| Zheng Wang | |
|---|---|
| contact affiliation | Jinan University |
| contact email | wangzheng@aierchina.com |
| lab head | |
| Yuexi Chen | |
| contact affiliation | Jinan University |
| contact email | chenyuexi@stu2022.jnu.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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