PXD060831-1

PXD060831 is an original dataset announced via ProteomeXchange.

Title	Lamin A/C-EZH2 interaction in breast cancer cells
Description	Epithelial-to-mesenchymal transition (EMT) is a developmentally conserved cell biological program adopted by cancer cells to metastasize to different organs. In this process, compact, cobblestone-shaped epithelial cells transform into more slender-shaped mesenchymal cells. Throughout metastasis, cells reside at various stages along the E-M spectrum. Epithelial cells are not necessarily converted to mesenchymal cells, as cells in the E-M continuum exhibit greater survival advantages and better metastatic ability than complete mesenchymal forms. During metastasis, epithelial cells traverse through narrow capillaries, inducing nucleus rupture. The nucleus is protected by type V intermediate filament proteins, Lamins, in the inner nuclear membrane, providing structural integrity, facilitating genome organization, and acting as an impediment to cell migration. Therefore, Lamin expression is downregulated to facilitate nuclear compliance during EMT, serving as a precursor for metastasis. Here, we aim to address the mechanism of Lamin downmodulation and its role in genome organization during EMT. We have attempted to address these questions in three independent and well-established breast cancer cell lines representing cells at specific stages of the EM spectrum, providing a paradigm to elucidate the role of Lamins in genome organization and function during EMT.
HostingRepository	PRIDE
AnnounceDate	2025-12-23
AnnouncementXML	Submission_2025-12-22_22:46:35.561.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kundan Sengupta
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-14 09:07:58	ID requested
⏵ 1	2025-12-22 22:46:36	announced

Dataset with its publication pending

submitter keyword: breast cancer, EMT,Lamin A/C, EZH2

Kundan Sengupta
contact affiliation	Professor, Department of Biology, Indian Institute of Science Education and Research, Pune-411008, Maharashtra
contact email	kunsen@iiserpune.ac.in
lab head
Kundan Sengupta
contact affiliation	Professor
contact email	cbliiserpune@acads.iiserpune.ac.in
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060831
     1. Label: PRIDE project
     2. Name: Lamin A/C-EZH2 interaction in breast cancer cells