PXD060817-1

PXD060817 is an original dataset announced via ProteomeXchange.

Title	ExPEC OmpT LL-37 Cleavage LC-MS
Description	Colicin V (ColV)-like plasmids (ColVLPs) are virulence plasmids frequently carried by extra-intestinal pathogenic E. coli (ExPEC) that cause human and avian infection. ColVLPs encode OmpTp, an outer membrane protease with the capacity to cleave the antimicrobial peptide LL-37, which is expressed in the human urinary tract. Homologs of OmpTp also exist on the E. coli chromosome, and differences in enzyme activity and cleavage profiles are largely uncharacterised. For this section of the project, the capacity of each OmpT variant to cleave LL-37 is tested, by incubating isogenic E. coli strains expressing each OmpT homolog with LL-37, and subsequently using LC-MS to quantify LL-37 cleavage products.
HostingRepository	PRIDE
AnnounceDate	2025-11-21
AnnouncementXML	Submission_2025-11-20_19:53:32.004.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zheng Jie Lian
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: NEWT:562;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-14 04:53:32	ID requested
⏵ 1	2025-11-20 19:53:32	announced

Dataset with its publication pending

submitter keyword: LL-37, E coli, ExPEC, OmpT

Mark Schembri
contact affiliation	Institute for Molecular Bioscience, The University of Queensland
contact email	m.schembri@uq.edu.au
lab head
Zheng Jie Lian
contact affiliation	The University of Queensland, Australia
contact email	uqzlian@uq.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD060817

PRIDE project URI

• PRIDE
  1. PXD060817
     1. Label: PRIDE project
     2. Name: ExPEC OmpT LL-37 Cleavage LC-MS